miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells

Anwar Abdurahman; Wusimanjiang Aierken; Fei Zhang; Rahmantay Obulkasim; Jueken Aniwashi; Ablat Sulayman

doi:10.3389/fgene.2022.989912

miR-1306 induces cell apoptosis by targeting BMPR1B gene in the ovine granulosa cells

Front Genet. 2022 Sep 23:13:989912. doi: 10.3389/fgene.2022.989912. eCollection 2022.

Authors

Anwar Abdurahman^{1

2

3}, Wusimanjiang Aierken⁴, Fei Zhang⁵, Rahmantay Obulkasim⁶, Jueken Aniwashi⁷, Ablat Sulayman⁸

Affiliations

¹ Shenzhen Key Laboratory of Marine Bioresources and Ecology, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, China.
² College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
³ College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
⁴ The Fifth Affiliate Hospital of Xinjiang Medical University, Urumqi, China.
⁵ Animal Diseases Control and Prevention Centre of Xinjiang Uygur Autonomous Region, Urumqi, China.
⁶ Ili Kazak Autonomous Prefecture Institute of Animal Science, Ili, China.
⁷ College of Animal Science and Technology, Xinjiang Agricultural University, Urumqi, China.
⁸ Institute of Animal Husbandry, Xinjiang Academy of Animal Science, Urumqi, China.

Abstract

Bone morphogenetic protein receptor type-1B (BMPR1B) is one of the major gene for sheep prolificacy. However, few studies investigated its regulatory region. In this study, we reported that miR-1306 is a direct inhibitor of BMPR1B gene in the ovine granulosa cells (ovine GCs). We detected a miRNA response element of miR-1306 in the 3' untranslated region of the ovine BMPR1B gene. Luciferase assay showed that the ovine BMPR1B gene is a direct target of miR-1306. qPCR and western blotting revealed that miR-1306 reduces the expression of BMPR1B mRNA and protein in the ovine granulosa cells. Furthermore, miR-1306 promoted cell apoptosis by suppressing BMPR1B expression in the ovine granulosa cells. Overall, our results suggest that miR-1306 is an epigenetic regulator of BMPR1B, and may serve as a potential target to improve the fecundity of sheep.

Keywords: BMPR1B; apoptosis; granulosa cell; miR-1306; sheep.