Impact of lactisole on the time-intensity profile of selected sweeteners in dependence of the binding site

Corinna M Deck; Maik Behrens; Martin Wendelin; Jakob P Ley; Gerhard E Krammer; Barbara Lieder

doi:10.1016/j.fochx.2022.100446

Impact of lactisole on the time-intensity profile of selected sweeteners in dependence of the binding site

Food Chem X. 2022 Sep 16:15:100446. doi: 10.1016/j.fochx.2022.100446. eCollection 2022 Oct 30.

Authors

Corinna M Deck^{1

2}, Maik Behrens³, Martin Wendelin⁴, Jakob P Ley⁵, Gerhard E Krammer⁵, Barbara Lieder^{1

2}

Affiliations

¹ Christian Doppler Laboratory for Taste Research, Faculty of Chemistry, University of Vienna, Austria.
² Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, Austria.
³ Leibniz-Institute for Food Systems Biology at the Technical University of Munich, Freising, Germany.
⁴ Symrise Distribution GmbH, Vienna, Austria.
⁵ Symrise AG, Holzminden, Germany.

Abstract

Currently, there is limited insight into the influence of the different binding sites of agonists and antagonists of the sweet taste receptor TAS1R2/TAS1R3 on temporal sensory properties of sweet tasting compounds. We investigated whether the binding site and a competitive or allosteric inhibition of TAS1R2/TAS1R3 influence the time-dependent sensory perception and in vitro TAS1R2/TAS1R3-activation profiles. We compared time-intensity ratings of cyclamate, NHDC, acesulfame K, and aspartame with and without lactisole with the corresponding TAS1R2/TAS1R3-activation in transfected HEK293 cells. In combination with lactisole, cyclamate and NHDC demonstrated a shift of the dose-response curve corresponding to a competitive inhibition by lactisole in the sensory and the cell experiments. Allosteric inhibition by lactisole for aspartame and acesulfame K was seen in the cell experiments, but not the sensory ratings. In conclusion, the data do not support a major impact of the binding site on the time-intensity profile of the tested sweeteners.

Keywords: Acesulfame K; Aspartame; Cyclamate; NHDC; Sweet taste receptor; Time-intensity.