Protocols for genetic labeling and tracing of Staphylococcus xylosus during tumor progression

Tingting Dong; Aikun Fu; Shang Cai

doi:10.1016/j.xpro.2022.101624

Protocols for genetic labeling and tracing of Staphylococcus xylosus during tumor progression

STAR Protoc. 2022 Dec 16;3(4):101624. doi: 10.1016/j.xpro.2022.101624. Epub 2022 Oct 7.

Authors

Tingting Dong¹, Aikun Fu², Shang Cai³

Affiliations

¹ Fudan University, Shanghai, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China. Electronic address: dongtingting@westlake.edu.cn.
² Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China.
³ Fudan University, Shanghai, China; Westlake Laboratory of Life Sciences and Biomedicine, Hangzhou, Zhejiang, China; School of Life Sciences, Westlake University, Hangzhou, Zhejiang, China. Electronic address: caishang@westlake.edu.cn.

Abstract

Intratumor microbiota is a dynamic cancer component that can be carried over by metastatic tumor cells to distal organs. This protocol was developed to genetically label Staphylococcus xylosus and trace the recombinant strain in vivo in the tumor. We optimized the recombination-based gene replacement protocol to insert a GFP-Erythromycin resistant protein (Erm) cassette. The inserted cassette facilitates the tracking of the recombinant strain, allowing a sensitive interrogation of microbial dynamics with high temporal and spatial resolution. For complete details on the use and execution of this protocol, please refer to Fu et al. (2022).

Keywords: Cancer; Microbiology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Erythromycin* / metabolism
Proteins / metabolism
Staphylococcus* / genetics
Staphylococcus* / metabolism

Substances

Erythromycin
Proteins

Supplementary concepts

Staphylococcus xylosus