Objective: To investigate the effects of curcumin on the proliferation, apoptosis, and cell cycle of human acute myeloid leukemia cell line K562.
Methods: MTT method was used to detect the proliferation inhibition of logarithmic growth phase human acute myeloid leukemia K562 cells, flow cytometry was used to detect the cell cycle, Annexin V-FITC was used to detect the apoptosis rate, and real-time fluorescent quantitative PCR and Western blot were used to detect the expression of Bax, BCL-2 and caspase-3 mRNA and protein, respectively.
Results: The inhibition rate of cell proliferation in curcumin 10, 20, and 40 μmol/L group for 24 h and 48 h were higher than that in the control group (curcumin 0 μmol/L), and the cell proliferation inhibition rate was concentration-time dependent (r=0.879, r=0.914). The proportion of G0/G1 cells and apoptosis rate of K562 cells in the curcumin 10, 20, and 40 μmol/L group were higher than those in the control group, and showed drug concentration dependent (r=0.856, r=0.782). The expression of Bax and Caspase-3 mRNA in the curcumin 10, 20, and 40 μmol/L group was higher, while BCL-2 mRNA was lower than those in the control group, and showed drug concentration dependent (r=0.861, r=0.748, r=-0.817). The gray value of Bax protein expression in the curcumin 10, 20, and 40 μmol/L group was higher than that in the control group, while the gray value of BCL-2 and Caspase-3 protein expression was lower than that in the control group, and showed drug concentration dependent (r=0.764, r=-0.723, r=-0.831).
Conclusion: Curcumin can inhibit the proliferation of human acute myeloid leukemia cell line K562 cells, block the cell cycle at G0/G1 phase, promote cell apoptosis, and induce apoptosis by regulating Bax, BCL-2, and Caspase-3.
题目: 姜黄素对人急性髓系白血病细胞株K562增殖、凋亡及细胞周期的影响.
目的: 探讨姜黄素对人急性髓系白血病细胞株K562增殖、凋亡及细胞周期的影响。.
方法: 取对数生长期人急性髓系白血病K562细胞，用MTT法测定细胞增殖抑制情况，流式细胞术检测细胞周期，Annexin V-FITC检测细胞凋亡率，实时荧光定量PCR法和Western blot分别检测Bax、BCL-2和Caspase-3 mRNA和蛋白的表达。.
结果: 姜黄素10、20和40 μmol/L组培养24 h和48 h细胞增殖抑制率均高于对照组（姜黄素0 μmol/L），且细胞增殖抑制率呈浓度-时间依赖性（r=0.879，r=0.914）。姜黄素10、20和40 μmol/L组G0/G1细胞比例和细胞凋亡率均高于对照组，且呈药物浓度依赖性（r=0.856，r=0.782）。姜黄素10、20和40 μmol/L组Bax和Caspase-3 mRNA表达高于对照组，而BCL-2 mRNA表达低于对照组，且呈药物浓度依赖性（r=0.861，r=0.748，r=-0.817）。姜黄素10、20和40 μmol/L组Bax蛋白表达灰度值高于对照组，而BCL-2和Caspase-3蛋白表达灰度值低于对照组，且呈药物浓度依赖性（r=0.764，r=-0.723，r=-0.831）。.
结论: 姜黄素可抑制人急性髓系白血病细胞株K562细胞增殖，将细胞周期阻滞于G0/G1期，促进细胞凋亡，且通过调控Bax、BCL-2和Caspase-3而诱导K562细胞凋亡。.
Keywords: K562 cells; acute myeloid leukemia; cell cycle; apoptosis; curcumin; proliferation.