Applications of acellular human amniotic membrane in regenerative medicine

Shaghayegh Doudi; Mansoureh Barzegar; Elham Angouraj Taghavi; Mohammad Eini; Arian Ehterami; Karen Stokes; Jonathan S Alexander; Majid Salehi

doi:10.1016/j.lfs.2022.121032

Applications of acellular human amniotic membrane in regenerative medicine

Life Sci. 2022 Dec 1:310:121032. doi: 10.1016/j.lfs.2022.121032. Epub 2022 Oct 5.

Authors

Shaghayegh Doudi¹, Mansoureh Barzegar², Elham Angouraj Taghavi³, Mohammad Eini⁴, Arian Ehterami⁵, Karen Stokes⁶, Jonathan S Alexander⁷, Majid Salehi⁸

Affiliations

¹ Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. Electronic address: doodi@shmu.ac.ir.
² Department of Ophthalmology, Wilmer Eye Institute, Johns Hopkins School of Medicine, Baltimore, MD, USA; Department of Molecular and Cellular Physiology, Louisiana State University Health Science Center-Shreveport, LA, USA. Electronic address: mbarzeg1@jhu.edu.
³ Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran. Electronic address: elhamtaghavi76@shmu.ac.ir.
⁴ Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran.
⁵ Institute for Regenerative Medicine (IREM), University of Zurich, Zurich, Switzerland. Electronic address: Arian.ehterami@irem.uzh.ch.
⁶ Department of Molecular and Cellular Physiology, Louisiana State University Health Science Center-Shreveport, LA, USA. Electronic address: karen.stokes@Isuhs.edu.
⁷ Department of Molecular and Cellular Physiology, Louisiana State University Health Science Center-Shreveport, LA, USA. Electronic address: Jonathan.alexander@Isuhs.edu.
⁸ Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran; Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran; Sexual Health and Fertility Research Center, Shahroud University of Medical Sciences, Shahroud, Iran. Electronic address: salehi.m@shmu.ac.ir.

PMID: 36206834
DOI: 10.1016/j.lfs.2022.121032

Abstract

The human amniotic membrane (hAM) surrounds and protects the fetus. It mainly consists of a thick basement membrane and an avascular stromal matrix. The structural, physical, and biological properties of AM make it a wonderful choice to use in regenerative medicine applications. This review discusses recent applications of hAM in skin transplantation and wound healing, ophthalmology, orthopedics, cell culture matrix, cell delivery system, cardiac injury, male and female reproductive systems, and liver. Moreover, an overview is presented on the advantages and disadvantages of using hAM and some methods to systematically refine and standardize hAM preparation.

Keywords: Acellular; Amniotic membrane; Regenerative medicine.

Publication types

Review

MeSH terms

Amnion*
Cell Culture Techniques
Female
Humans
Male
Regenerative Medicine*
Wound Healing