Visualization of Exo- and Endocytosis Membrane Dynamics with Super-Resolution STED Microscopy

Chung Yu Chan; Sue Han; Xin Wang; Xiaoli Guo; Ling-Gang Wu

doi:10.1007/978-1-0716-2671-9_6

Visualization of Exo- and Endocytosis Membrane Dynamics with Super-Resolution STED Microscopy

Methods Mol Biol. 2023:2565:77-87. doi: 10.1007/978-1-0716-2671-9_6.

Authors

Chung Yu Chan¹, Sue Han², Xin Wang², Xiaoli Guo², Ling-Gang Wu³

Affiliations

¹ National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. keith.chan@nih.gov.
² National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA.
³ National Institute of Neurological Disorders and Stroke, Bethesda, MD, USA. wul@ninds.nih.gov.

PMID: 36205888
DOI: 10.1007/978-1-0716-2671-9_6

Abstract

Recent advances in stimulated emission depletion (STED) microscopy offer an unparalleled avenue to study membrane dynamics of exo- and endocytosis, such as fusion pore opening, pore expansion, constriction, and closure, as well as the membrane transformation from flat-shaped to round-shaped vesicles in real time. Here we depict a method of using the state-of-the-art STED microscopy to image these membrane dynamics in bovine chromaffin cells. This method can potentially be applied to study other membrane structure dynamics in other cell model system.

Keywords: Endocytosis; Exocytosis; Fusion pore; STED microscopy.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Animals
Cattle
Cell Membrane / metabolism
Chromaffin Cells*
Endocytosis
Microscopy*
Secretory Vesicles / metabolism

Abstract

Publication types

MeSH terms

Grants and funding