Immunocytochemistry of Acutely Isolated Adrenal Medullary Chromaffin Cells

Keita Harada; Hidetada Matsuoka; Masumi Inoue

doi:10.1007/978-1-0716-2671-9_3

Immunocytochemistry of Acutely Isolated Adrenal Medullary Chromaffin Cells

Methods Mol Biol. 2023:2565:35-42. doi: 10.1007/978-1-0716-2671-9_3.

Authors

Keita Harada¹, Hidetada Matsuoka², Masumi Inoue³

Affiliations

¹ Department of Cell and Systems Physiology, University of Occupational and Environmental Health School of Medicine, Kitakyushu, Japan.
² Yokohama University of Pharmacy, Yokohama, Japan.
³ University of Occupational and Environmental Health School of Medicine, Kitakyushu, Japan. minoue@med.uoeh-u.ac.jp.

PMID: 36205885
DOI: 10.1007/978-1-0716-2671-9_3

Abstract

Immunocytochemistry enables the detection and localization of proteins in cells that are acutely dissociated or in culture. There are advantages and disadvantages to the use of cultured cells for immunocytochemistry. One of the advantages is that cultured cells can be used for one or more weeks after the dissociation of cells, whereas one of the disadvantages is that the properties of cells in culture might change under artificial conditions. On the other hand, acutely dissociated cells are expected to have the original properties of cells because almost all procedures before fixation, except for enzymatic digestion, are carried out at low temperatures. Here, we describe how adrenal medullary cells of small animals are acutely dissociated for immunostaining.

Keywords: Acutely isolated cell; Adrenal medullary chromaffin cell; Confocal microscopy; Immunocytochemistry; Immunofluorescence.

MeSH terms

Adrenal Medulla*
Animals
Cells, Cultured
Chromaffin Cells*
Endocrine Cells*
Immunohistochemistry