Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin

Andrei V Gannesen; Rustam H Ziganshin; Evelina L Zdorovenko; Alena I Klimko; Elena A Ianutsevich; Olga A Danilova; Vera M Tereshina; Maxim V Gorbachevskii; Maria A Ovcharova; Ekaterina D Nevolina; Sergey V Martyanov; Alexander S Shashkov; Andrey S Dmitrenok; Andrei A Novikov; Marina V Zhurina; Ekaterina A Botchkova; Philipp V Toukach; Vladimir K Plakunov

doi:10.3389/fmicb.2022.1003942

Epinephrine extensively changes the biofilm matrix composition in Micrococcus luteus C01 isolated from human skin

Front Microbiol. 2022 Sep 20:13:1003942. doi: 10.3389/fmicb.2022.1003942. eCollection 2022.

Authors

Andrei V Gannesen¹, Rustam H Ziganshin², Evelina L Zdorovenko³, Alena I Klimko⁴, Elena A Ianutsevich¹, Olga A Danilova¹, Vera M Tereshina¹, Maxim V Gorbachevskii⁵, Maria A Ovcharova¹, Ekaterina D Nevolina¹, Sergey V Martyanov¹, Alexander S Shashkov³, Andrey S Dmitrenok³, Andrei A Novikov⁵, Marina V Zhurina¹, Ekaterina A Botchkova^{1

5}, Philipp V Toukach³, Vladimir K Plakunov¹

Affiliations

¹ Winogradsky Institute of Microbiology, Federal Research Center "Fundamentals of Biotechnology" of Russian Academy of Sciences, Moscow, Russia.
² Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia.
³ N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.
⁴ Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
⁵ Faculty of Chemical and Environmental Engineering, Gubkin University, Moscow, Russia.

Abstract

The importance of the impact of human hormones on commensal microbiota and microbial biofilms is established in lots of studies. In the present investigation, we continued and extended the research of epinephrine effects on the skin commensal Micrococcus luteus C01 and its biofilms, and also the matrix changes during the biofilm growth. Epinephrine in concentration 4.9 × 10^-9 M which is close to normal blood plasma level increased the amount of polysaccharides and extracellular DNA in the matrix, changed extensively its protein, lipid and polysaccharide composition. The Ef-Tu factor was one of the most abundant proteins in the matrix and its amount increased in the presence of the hormone. One of the glucose-mannose polysaccharide was absent in the matrix in presence of epinephrine after 24 h of incubation. The matrix phospholipids were also eradicated by the addition of the hormone. Hence, epinephrine has a great impact on the M. luteus biofilms and their matrix composition, and this fact opens wide perspectives for the future research.

Keywords: Micrococcus luteus; NMR; biofilm matrix; biofilms; epinephrine; host–microbiota interactions; human skin microbiota; mass spectrometry.

Copyright © 2022 Gannesen, Ziganshin, Zdorovenko, Klimko, Ianutsevich, Danilova, Tereshina, Gorbachevskii, Ovcharova, Nevolina, Martyanov, Shashkov, Dmitrenok, Novikov, Zhurina, Botchkova, Toukach and Plakunov.