Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani

Xiangru Chen; Zhaoyao Yu; Yujia Sun; Meipeng Yang; Ning Jiang

doi:10.3389/fmicb.2022.978075

Molecular characterization of a novel partitivirus isolated from Rhizoctonia solani

Front Microbiol. 2022 Sep 20:13:978075. doi: 10.3389/fmicb.2022.978075. eCollection 2022.

Authors

Xiangru Chen¹, Zhaoyao Yu¹, Yujia Sun¹, Meipeng Yang¹, Ning Jiang²

Affiliations

¹ Key Laboratory of Agricultural Microbiology, College of Agriculture, Guizhou University, Guiyang, China.
² Agronomic Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, China.

Abstract

Rhizoctonia solani is a widely distributed plant pathogen that can damage many crops. Here, we identified a novel mycovirus tentatively named Rhizoctonia solani partitivirus 433 (RsPV433) from an R. solani (AG-3) strain which caused tobacco target spot disease on flue-cured tobacco. RsPV433 was consisted of two dsRNA segments with lengths of 2450 and 2273 bp, which encoded an RNA-dependent RNA polymerase and a coat protein, respectively. BLASTP results of RsPV433 showed that the closest relative of RsPV433 was Sarcosphaera coronaria partitivirus (QLC36830.1), with an identity of 60.85% on the RdRp amino sequence. Phylogenetic analysis indicated that RsPV433 belonged to the Betapartitivirus genus in the Partitiviridae family. The virus transmission experiment revealed that RsPV433 can be transmitted horizontally. We further tested the biological effect of RsPV433 on R. solani strains and found that the RsPV433-infected R. solani strain grew slower than the RsPV433-free strain on the PDA medium and RsPV433 seemed to have no obvious impact on the lesion inducing ability of R. solani.

Keywords: Partitiviridae; Rhizoctonia solani; dsRNA (double-stranded RNA); mycovirus; tobacco target spot disease.