20-HETE/GPR75 pairing modulates the expression and transcriptional activity of the androgen receptor in androgen-sensitive prostate cancer cells

Mol Cell Endocrinol. 2023 Jan 1:559:111784. doi: 10.1016/j.mce.2022.111784. Epub 2022 Oct 3.

Abstract

The androgen receptor (AR) and AR-driven genes are crucial in normal and neoplastic prostate tissue. Previous results showed a link between 20-hydroxyeicosatetraenoic acid (20-HETE) production and AR-driven prostate cancer (PCa) progression. This study aims to describe the contribution of GPR75, 20-HETE membrane receptor, in 20-HETE-mediated expression and transcriptional activity of AR in PCa. In LNCaP cells, 20-HETE increased AR expression, nuclear localization, and its transcriptional activity. Also, 20-HETE enhanced dihydrotestosterone (DHT) induced effects. All was abrogated by chemical antagonism of GPR75 (19-HEDE) or its transient knockdown. In human PCa, the expression of AR-driven genes correlated with GPR75. In LNCaP xenografts, tumors from castrated animals expressed higher levels of AR, this was impaired by inhibition of 20-HETE synthesis. These data suggest that 20-HETE, through the GPR75 receptor, regulates transcriptionally active AR in PCa cells, thus making 20-HETE/GRP75 potential targets to limit the expression of AR-driven phenotype in PCa cells.

Keywords: 20-HETE; Androgen receptor; GPR75; Prostate cancer; Tumorigenesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Androgens / metabolism
  • Androgens / pharmacology
  • Animals
  • Cell Line, Tumor
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Male
  • Prostate / metabolism
  • Prostatic Neoplasms* / metabolism
  • Receptors, Androgen* / metabolism
  • Receptors, G-Protein-Coupled / metabolism

Substances

  • Receptors, Androgen
  • 20-hydroxy-5,8,11,14-eicosatetraenoic acid
  • Androgens
  • GPR75 protein, human
  • Receptors, G-Protein-Coupled