Microfluidic biosensor for one-step detection of multiplex foodborne bacteria ssDNA simultaneously by smartphone

Gaowa Xing; Nan Li; Haifeng Lin; Yuting Shang; Qiaosheng Pu; Jin-Ming Lin

doi:10.1016/j.talanta.2022.123980

Microfluidic biosensor for one-step detection of multiplex foodborne bacteria ssDNA simultaneously by smartphone

Talanta. 2023 Feb 1:253:123980. doi: 10.1016/j.talanta.2022.123980. Epub 2022 Sep 30.

Authors

Gaowa Xing¹, Nan Li², Haifeng Lin², Yuting Shang², Qiaosheng Pu³, Jin-Ming Lin⁴

Affiliations

¹ College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China; Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
² Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China.
³ College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, China. Electronic address: puqs@lzu.edu.cn.
⁴ Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Department of Chemistry, Tsinghua University, Beijing, 100084, China. Electronic address: jmlin@mail.tsinghua.edu.cn.

PMID: 36201954
DOI: 10.1016/j.talanta.2022.123980

Abstract

As a major threat to food safety due to their pathogenicity, foodborne bacteria have received much attention. In this paper, we present a one-step and wash-free microfluidic biosensor platform by smartphone for simultaneous multiple foodborne bacteria target single-stranded DNA (ssDNA) detection. This technology is based on the fluorescence resonance energy transfer (FRET) between the graphene oxide (GO) and fluorescence molecules modified capture ssDNA of the target bacteria ssDNA (ctDNA) which were coated on the microfluidic chips. The fluorescence recovery was recorded by a smartphone fluorescent detector. With an optimal analytical performance, the platform realized the detection of four kinds of bacteria ssDNA simultaneously within 5 min, with the limits of detection (LODs) of 0.17, 0.18, 0.27, and 0.17 nM, respectively. And the throughput analysis of trace amounts of foodborne bacteria ssDNA in milk and water samples were successfully detected. This one-step and wash-free microfluidic biosensor can be used as a tool for food safety analysis.

Keywords: FRET; Foodborne bacteria; Microfluidic biosensor; Multiplex detection; One-step detection.

MeSH terms

Bacteria
DNA, Single-Stranded*
Microfluidics*

Substances

DNA, Single-Stranded