A portable smartphone-based detection of glyphosate based on inhibiting peroxidase-like activity of heptanoic acid/Prussian blue decorated Fe3O4 nanoparticles

Dan Chen; Chunqiong Wang; Dezhi Yang; Huimin Deng; Qiulan Li; Li Chen; Gaokun Zhao; Junli Shi; Ke Zhang; Yaling Yang

doi:10.1039/d2ra03382h

A portable smartphone-based detection of glyphosate based on inhibiting peroxidase-like activity of heptanoic acid/Prussian blue decorated Fe₃O₄ nanoparticles

RSC Adv. 2022 Sep 5;12(38):25060-25067. doi: 10.1039/d2ra03382h. eCollection 2022 Aug 30.

Authors

Dan Chen^{1

2}, Chunqiong Wang², Dezhi Yang³, Huimin Deng⁴, Qiulan Li³, Li Chen⁵, Gaokun Zhao⁶, Junli Shi⁶, Ke Zhang², Yaling Yang³

Affiliations

¹ Peking University, School of Materials Science and Engineering Beijing 100871 China.
² Yunnan Institute of Tobacco Quality Inspection & Supervision Kunming 650500 China swukirk@126.com.
³ Faculty of Life Science and Technology, Kunming University of Science and Technology Kunming 650500 China yangyl2016@qq.com.
⁴ China National Tobacco Quality Supervision & Test Center Zhengzhou 450001 China.
⁵ Zhengzhou Tobacco Research Institute of CNTC Zhengzhou China.
⁶ Yunnan Academy of Tobacco Agricultural Sciences Kunming 650021 China.

Abstract

The rapid and onsite detection of glyphosate in tobacco products is still a great challenge. In this study, a novel smartphone-assisted sensing platform for the detection of glyphosate has been successfully proposed through the peroxidase-like activity of Fe₃O₄-based nanozyme. Heptanoic acid/Prussian blue (PB) decorated Fe₃O₄ nanoparticles (Fe₃O₄@C₇/PB) could catalyze and oxidize 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS, colorless) into a steel blue colored product in the presence of hydrogen peroxide. Glyphosate could specifically inhibit the peroxidase-like activity of Fe₃O₄@C₇/PB by occupying the active site, thereby the glyphosate detection could be accomplished within 10 min by monitoring the color change of ABTS. This study has developed a smartphone-based portable detection platform for online analysis of glyphosate with a detection limit of 0.1 μg mL^-1. The absorbance response curve of glyphosate showed good linearity in the concentration range of 0.125-15 μg mL^-1 at 415, 647, and 730 nm. Moreover, by employing a co-precipitation technology and inhibiting the peroxidase-like activity, the glyphosate analysis would be less affected by the tobacco sample matrix. The nanosensor possesses excellent selectivity and anti-interference ability, which has application value in actual samples for onsite screening.