Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum

Xiaojing Lv; Chunli Jiao; Yuying Chen; Lizhu Chen; Xuan Li; Xiangquan Liu; Dinglong Yang; Jianmin Zhao

doi:10.1016/j.fsi.2022.09.068

Characteristic and antibacterial effect of a histone H2A and its preliminary roles in extracellular traps in manila clam Ruditapes philippinarum

Fish Shellfish Immunol. 2022 Dec:131:77-83. doi: 10.1016/j.fsi.2022.09.068. Epub 2022 Oct 3.

Authors

Xiaojing Lv¹, Chunli Jiao², Yuying Chen³, Lizhu Chen³, Xuan Li³, Xiangquan Liu³, Dinglong Yang⁴, Jianmin Zhao⁵

Affiliations

¹ Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China; Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China; University of Chinese Academy of Sciences, Beijing, 100049, PR China.
² Yantai Center for Food and Drug Control, Yantai, 264003, PR China.
³ Shandong Key Laboratory of Marine Ecological Restoration, Shandong Marine Resources and Environment Research Institute, Yantai, 264006, PR China.
⁴ Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China; Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China; Center for Ocean Mega-science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, PR China. Electronic address: dlyang@yic.ac.cn.
⁵ Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China; Research and Development Center for Efficient Utilization of Coastal Bioresources, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, PR China; Center for Ocean Mega-science, Chinese Academy of Sciences, Qingdao, Shandong, 266071, PR China.

PMID: 36195271
DOI: 10.1016/j.fsi.2022.09.068

Abstract

In the present study, a histone H2A (designed as RpH2A) was identified and characterized from clam Ruditapes philippinarum, and its open reading frame (ORF) was of 387 bp encoding a polypeptide of 128 amino acids. The deduced amino acid sequence of RpH2A shared high identities ranging from 57.1% to 96.1% with that of other identified H2A. The mRNA expression of RpH2A was up-regulated significantly after Vibrio anguillarum challenge. The recombinant RpH2A protein (rRpH2A) displayed significantly binding affinity to lipopolysaccharide (LPS) and peptidoglycan (PGN) in vitro, and also exhibited antimicrobial properties against Escherichia coli. In addition, the antimicrobial RpH2A was shown to co-localize with extracellular traps (ETs) released from hemocytes induced by E. coli, suggesting that RpH2A might contribute to eliminate invading bacteria in clam ETs. Altogether, our data revealed that RpH2A could function as antimicrobial peptides, which might play a crucial role in the immune responses of hemocytes ETs in clams.

Keywords: Antimicrobial activity; Extracellular traps; Histone; Ruditapes philippinarum.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Bivalvia*
Escherichia coli
Extracellular Traps*
Histones
Vibrio* / physiology

Substances

Histones
Anti-Bacterial Agents