Description and complete mitochondrial genome of Atkinsoniella zizhongi sp. nov. (Hemiptera: Cicadellidae: Cicadellinae) from China and its phylogenetic implications

PeerJ. 2022 Sep 28:10:e14026. doi: 10.7717/peerj.14026. eCollection 2022.

Abstract

A new species, Atkinsoniella zizhongi sp. nov. of the subfamily Cicadellinae, was described and illustrated from China. The new species is similar to A. nigrominiatula (Jacobi, 1944), A. limba Kuoh, 1991, A. dormana Li, 1992, A. peaka Yang, Meng et Li, 2017, and A. divaricata Yang, Meng et Li, 2017. But the characteristics of aedeagus and pygofer process can be used to distinguish them easily. The complete mitochondrial genome of the paratype was sequenced and assembled. The mitogenome of A. zizhongi sp. nov. was 16,483 bp in length, with an A+T content of 75.9%, containing 37 typical genes and a control region (CR). The gene order was consistent with the inferred insect ancestral mitochondrial genome. All of the PCGs were determined to have the typical stop codon TAA or TAG, while COX2 and ND5 ended with incomplete termination codons T and TA, respectively. In addition, phylogenetic trees were reconstructed based on PCGs and rRNAs using both the maximum likelihood (ML) and Bayesian inference (BI) methods. The results showed that the intergeneric and interspecific relationships within the subfamily Cicadellinae were completely consistent in all of the phylogenetic trees, except that the different interspecific relationships within the genus Bothrogonia were detected in the ML analysis based on the amino acid sequences. This study enriches the species diversity of Cicadellinae and further promotes research on its phylogeny.

Keywords: Cicadellinae; Hemiptera; Mitogenome; New species; Phylogenetics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Bayes Theorem
  • Genome, Mitochondrial* / genetics
  • Hemiptera*
  • Phylogeny

Grants and funding

This research was supported by the Guizhou Province Science and Technology Innovation Talent Team Project (No. Qian Ke He Pingtai Rencai – CXTD [2021]004), the Guizhou Provincial Department of Education Youth Science and Technology Talents Growth Project (No. Qian Jiao He KY word [2018]103), the Introducing Talent Research Program for Guizhou University (No. Gui Da Ren Ji He word [2016]70); the National Nature Science Foundation of Guizhou University Cultivating Program (No. Qian Ke He Platform Talent [2017]5788-32), and the Graduate Innovation Fund, Guizhou Province, China (No. Qianjiaohe YJSCXJH [2019]023). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.