Absorption and transport of myofibrillar protein-bound Nɛ-(carboxymethyl)lysine in Caco-2 cells after simulated gastrointestinal digestion

Mengyu Ye; Xiaomin Qi; Xiaomin Ren; Wei Quan; Huaide Xu; Maomao Zeng; Jie Chen; Mei Li

doi:10.1016/j.foodres.2022.111870

Absorption and transport of myofibrillar protein-bound N^ɛ-(carboxymethyl)lysine in Caco-2 cells after simulated gastrointestinal digestion

Food Res Int. 2022 Nov:161:111870. doi: 10.1016/j.foodres.2022.111870. Epub 2022 Aug 27.

Authors

Mengyu Ye¹, Xiaomin Qi¹, Xiaomin Ren¹, Wei Quan², Huaide Xu¹, Maomao Zeng³, Jie Chen⁴, Mei Li⁵

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
² College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
⁴ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi 214122, China.
⁵ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. Electronic address: limei1101@nwafu.edu.cn.

PMID: 36192990
DOI: 10.1016/j.foodres.2022.111870

Abstract

This study aimed to investigate the absorption and transport of myofibrillar protein-bound N^ɛ-(carboxymethyl)lysine (MP-bound CML) in Caco-2 cells after simulated gastrointestinal digestion. Four kinds of MP-bound CML hydrolysates with molecular weights (MWs) less than 1 kDa, 1-3 kDa, 3-5 kDa and greater than 5 kDa, were obtained by ultrafiltration; their absorption and transport were studied in Caco-2 cells. Peptide-bound CML in hydrolysates with MWs less than 1 kDa was absorbed by 6.58 % and might transport across Caco-2 cells monolayer through paracellular pathway; peptide-bound CML in hydrolysates with MWs 1-3 kDa was absorbed by 12.8 % and might transport across Caco-2 cells monolayer through paracellular pathway and transcytosis route; peptide-bound CML in hydrolysates with MWs 3-5 kDa was absorbed by 14.66 % and might be through active route via PepT-1 transport across Caco-2 cells monolayer; whereas protein-bound CML in hydrolysates with MWs greater than 5 kDa was only absorbed by 1.02 %, which was hardly transported into Caco-2 cells. In conclusion, MP-bound CML could be absorbed by 35.06 % into Caco-2 cells after simulated gastrointestinal digestion and is transported across Caco-2 cells through paracellular pathway, transcytosis route and active route via PepT-1.

Keywords: Absorption; Caco-2 cell; Myofibrillar protein-bound N(ɛ)-(carboxymethyl)lysine; Simulated gastrointestinal digestion; Transport.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Transport
Caco-2 Cells
Digestion
Humans
Lysine* / metabolism
Organoplatinum Compounds
Peptides* / metabolism

Substances

Organoplatinum Compounds
Peptides
1,10-phenanthroline-platinum(II)-ethylenediamine
Lysine