Comparison of tissue distribution of free and protein bound Nɛ-carboxymethyllysine after long-term oral administration to mice

Xiaojin Yuan; Yating Bai; Jing Zhang; Ruohan Zhai; Chenxi Nie; Aobai Tu; Suchen Li; Zhifei Chen; Min Zhang; Juxiu Li

doi:10.1016/j.foodres.2022.111787

Comparison of tissue distribution of free and protein bound Nɛ-carboxymethyllysine after long-term oral administration to mice

Food Res Int. 2022 Nov:161:111787. doi: 10.1016/j.foodres.2022.111787. Epub 2022 Aug 24.

Authors

Xiaojin Yuan¹, Yating Bai¹, Jing Zhang², Ruohan Zhai¹, Chenxi Nie¹, Aobai Tu¹, Suchen Li¹, Zhifei Chen¹, Min Zhang¹, Juxiu Li³

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China.
² College of Horticulture, Northwest A&F University, Yangling 712100, China.
³ College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China. Electronic address: lijuxiu@nwsuaf.edu.cn.

PMID: 36192938
DOI: 10.1016/j.foodres.2022.111787

Abstract

Nɛ-Carboxymethyl-lysine (CML) is a primary advanced glycation end product that exists in the body and food as free and bound forms with different bioavailability and physiological effects. To compare the uptake, tissue distribution, and fecal excretion of dietary free and bound CML, free or bound CML were administered to healthy mice at 10 mg CML kg^-1 body weight per day for 12 weeks. The results demonstrated that free CML was significantly absorbed in serum and accumulated in the colon, ileum, lung, kidneys, heart, spleen, brain, and liver after intake of free and bound CML, whereas no statistical increase was found in the accumulation of bound CML in the serum, lung, spleen, kidneys, and liver. The colon was the main tissue for the accumulation of free and total CML. Moreover, the accumulation of free CML in tissues and organs was significantly correlated with free CML levels in serum. In conclusion, consumption of bound CML caused a higher uptake, accumulation, and fecal excretion of CML in the body than intake of free CML.

Keywords: Accumulation; Advanced glycation end products; Fecal excretion; High-performance liquid chromatography-tandem mass spectrometry; Nε-carboxymethyllysine; Uptake.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Administration, Oral
Animals
Glycation End Products, Advanced* / metabolism
Lysine* / analogs & derivatives
Lysine* / metabolism
Mice
Proteins / metabolism
Tissue Distribution

Substances

Glycation End Products, Advanced
Proteins
N(6)-carboxymethyllysine
Lysine