Sensitivity of phytoplankton, zooplankton and macroinvertebrates to hydrogen peroxide treatments of cyanobacterial blooms

Abstract

Addition of hydrogen peroxide (H₂O₂) is a promising method to acutely suppress cyanobacterial blooms in lakes. However, a reliable H₂O₂ risk assessment to identify potential effects on non-target species is currently hampered by a lack of appropriate ecotoxicity data. The aim of the present study was therefore to quantify the responses of a wide diversity of freshwater phytoplankton, zooplankton and macroinvertebrates to H₂O₂ treatments of cyanobacterial blooms. To this end, we applied a multifaceted approach. First, we investigated the 24-h toxicity of H₂O₂ to three cyanobacteria (Planktothrix agardhii, Microcystis aeruginosa, Anabaena sp.) and 23 non-target species (six green algae, eight zooplankton and nine macroinvertebrate taxa), using EC₅₀ values based on photosynthetic yield for phytoplankton and LC₅₀ values based on mortality for the other organisms. The most sensitive species included all three cyanobacterial taxa, but also the rotifer Brachionus calyciflores and the cladocerans Ceriodaphnia dubia and Daphnia pulex. Next, the EC₅₀ and LC₅₀ values obtained from the laboratory toxicity tests were used to construct a species sensitivity distribution (SSD) for H₂O₂. Finally, the species predicted to be at risk by the SSD were compared with the responses of phytoplankton, zooplankton and macroinvertebrates to two whole-lake treatments with H₂O₂. The predictions of the laboratory-based SSD matched well with the responses of the different taxa to H₂O₂ in the lake. The first lake treatment, with a relatively low H₂O₂ concentration and short residence time, successfully suppressed cyanobacteria without major effects on non-target species. The second lake treatment had a higher H₂O₂ concentration with a longer residence time, which resulted in partial suppression of cyanobacteria, but also in a major collapse of rotifers and decreased abundance of small cladocerans. Our results thus revealed a trade-off between the successful suppression of cyanobacteria at the expense of adverse effects on part of the zooplankton community. This delicate balance strongly depends on the applied H₂O₂ dosage and may affect the decision whether to treat a lake or not.

Keywords: Harmful algal blooms; Lake treatments; Macroinvertebrates; Rotifers; Species sensitivity distribution; Zooplankton.