CircRPPH1 promotes the stemness of gastric cancer cells by targeting miR-375/SLC7A11 axis

Environ Toxicol. 2023 Jan;38(1):115-125. doi: 10.1002/tox.23668. Epub 2022 Oct 3.

Abstract

This study mainly focuses on revealing the role of circRPPH1 in gastric cancer cell stemness. In vitro and in vivo experiments were performed to evaluate the effects of circRPPH1 on gastric cancer cell stemness. Luciferase reporter and RIP assays were implemented to reveal the underlying mechanisms. MiR-375 directly bound to circRPPH1 in gastric cancer cells. And circRPPH1 acted as an miR-375 sponge to positively regulate SLC7A11 expression, which has been confirmed to be the direct target of miR-375 in gastric cancer, and thus regulated ferroptosis. Moreover, circRPPH1 promoted the stemness of gastric cancer cells dependent on the miR-375/SLC7A11. This study provides a potential target for gastric cancer progression based on the circRPPH1/miR-375/SLC7A11 regulatory axis.

Keywords: CircRPPH1; Ferroptosis; MiR-375; SLC7A11; Stemness; gastric cancer.

MeSH terms

  • Amino Acid Transport System y+* / genetics
  • Amino Acid Transport System y+* / metabolism
  • Cell Line, Tumor
  • Ferroptosis*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • RNA, Circular* / genetics
  • Stomach Neoplasms* / genetics

Substances

  • Amino Acid Transport System y+
  • MicroRNAs
  • MIRN375 microRNA, human
  • SLC7A11 protein, human
  • RNA, Circular