Functional and Structural Brain Connectivity in Children With Bilateral Cerebral Palsy Compared to Age-Related Controls and in Response to Intensive Rapid-Reciprocal Leg Training

Diane L Damiano; James J Pekar; Susumu Mori; Andreia Vasconcellos Faria; X Ye; Elaine Stashinko; Christopher J Stanley; Katharine E Alter; Alec H Hoon; Eric M Chin

doi:10.3389/fresc.2022.811509

Functional and Structural Brain Connectivity in Children With Bilateral Cerebral Palsy Compared to Age-Related Controls and in Response to Intensive Rapid-Reciprocal Leg Training

Front Rehabil Sci. 2022 Apr 5:3:811509. doi: 10.3389/fresc.2022.811509. eCollection 2022.

Authors

Diane L Damiano¹, James J Pekar^{2

3}, Susumu Mori⁴, Andreia Vasconcellos Faria³, X Ye³, Elaine Stashinko^{4

5}, Christopher J Stanley¹, Katharine E Alter¹, Alec H Hoon^{4

5}, Eric M Chin^{4

5}

Affiliations

¹ Department of Rehabilitation Medicine, NIH, Bethesda, MD, United States.
² FM Kirby Research Center for Functional Brain Imaging, Kennedy Krieger Institute, Baltimore, MD, United States.
³ Russell H. Morgan Department of Radiology and Radiological Science, Johns Hopkins School of Medicine, Baltimore, MD, United States.
⁴ Johns Hopkins School of Medicine, Baltimore, MD, United States.
⁵ Kennedy Krieger Institute, Baltimore, MD, United States.

Abstract

Background: Compared to unilateral cerebral palsy (CP), less is known about brain reorganization and plasticity in bilateral CP especially in relation or response to motor training. The few trials that reported brain imaging results alongside functional outcomes include a handful of studies in unilateral CP, and one pilot trial of three children with bilateral CP. This study is the first locomotor training randomized controlled trial (RCT) in bilateral CP to our knowledge reporting brain imaging outcomes.

Methods: Objective was to compare MRI brain volumes, resting state connectivity and white matter integrity using DTI in children with bilateral CP with PVL and preterm birth history (<34 weeks), to age-related controls, and from an RCT of intensive 12 week rapid-reciprocal locomotor training using an elliptical or motor-assisted cycle. We hypothesized that connectivity in CP compared to controls would be greater across sensorimotor-related brain regions and that functional (resting state) and structural (fractional anisotropy) connectivity would improve post intervention. We further anticipated that baseline and post-intervention imaging and functional measures would correlate.

Results: Images were acquired with a 3T MRI scanner for 16/27 children with CP in the trial, and 18 controls. No conclusive evidence of training-induced neuroplastic effects were seen. However, analysis of shared variance revealed that greater increases in precentral gyrus connectivity with the thalamus and pons may be associated with larger improvements in the trained device speed. Exploratory analyses also revealed interesting potential relationships between brain integrity and multiple functional outcomes in CP, with functional connectivity between the motor cortex and midbrain showing the strongest potential relationship with mobility. Decreased posterior white matter, corpus callosum and thalamic volumes, and FA in the posterior thalamic radiation were the most prominent group differences with corticospinal tract differences notably not found.

Conclusions: Results reinforce the involvement of sensory-related brain areas in bilateral CP. Given the wide individual variability in imaging results and clinical responses to training, a greater focus on neural and other mechanisms related to better or worse outcomes is recommended to enhance rehabilitation results on a patient vs. group level.

Keywords: brain imaging; brain volumes; imaging; physical therapy; plasticity; spastic diplegia.