Ameliorative effect of bayberry leaves proanthocyanidins on high sugar diet induced Drosophila melanogaster

Mengting Wang; Haiguang Mao; Jianchu Chen; Lili Qi; Jinbo Wang

doi:10.3389/fphar.2022.1008580

Ameliorative effect of bayberry leaves proanthocyanidins on high sugar diet induced Drosophila melanogaster

Front Pharmacol. 2022 Sep 15:13:1008580. doi: 10.3389/fphar.2022.1008580. eCollection 2022.

Authors

Mengting Wang¹, Haiguang Mao¹, Jianchu Chen², Lili Qi¹, Jinbo Wang¹

Affiliations

¹ School of Biological and Chemical Engineering, NingboTech University, Ningbo, China.
² College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, China.

Abstract

Bayberry leaves proanthocyanidins (BLPs) were distributed in natural plant food, considered to have the potential for metabolic syndrome. In this study, we raised Drosophila melanogaster on high sugar diet (HSD) from the egg stage to induce hyperglycemia, and the ameliorative effect of BLPs was assessed based on this model. Phenotypical, biochemical, and molecular analyses related to diabetes mellitus pathogenesis were measured. Flies exposed to BLPs were found to suppress the HSD-induced high glucose and high triglycerides levels. Moreover, BLPs showed an inhibitory effect on carbohydrate digestive enzymes (α-amylase and α-glucosidase) activity and mRNA expression, exhibiting the potential for carbohydrate digestion retardation. Transcriptional levels of key genes associated with glycolipid metabolism were further evaluated, including dilp, InR, and downstream dAKT-dFOXO-PEPCK, together with E78, SREBP, FAS, and LSD genes, were all downregulated after BLPs-exposure, suggesting the ameliorative effect of BLPs on dysbiosis associated with the insulin signaling pathway. This study provided a new functional compound, which is beneficial to further antidiabetic therapy studies.

Keywords: bayberry leaves proanthocyanidins; drosophila; gene markers; hyperglycemia; physiological markers.