Tensile Strength of Porcine Aorta Decellularized with Liquefied Dimethyl Ether and DNase

Hideki Kanda; Kento Oya; Toshihira Irisawa; Wahyudiono; Motonobu Goto

doi:10.1021/acsomega.2c04103

Tensile Strength of Porcine Aorta Decellularized with Liquefied Dimethyl Ether and DNase

ACS Omega. 2022 Sep 13;7(38):34449-34453. doi: 10.1021/acsomega.2c04103. eCollection 2022 Sep 27.

Authors

Hideki Kanda¹, Kento Oya¹, Toshihira Irisawa², Wahyudiono^{1

3}, Motonobu Goto^{1

4}

Affiliations

¹ Department of Materials Process Engineering, Nagoya University, Nagoya, Aichi 464-8603 Japan.
² Department of Chemical Systems Engineering, Nagoya University, Nagoya, Aichi 464-8603 Japan.
³ New Industry Creation Hatchery Center, Tohoku University, 6-6-10 Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
⁴ Super Critical Technology Centre Co. Ltd., Kuwana, Mie 511-0838, Japan.

Abstract

In a previous report, we proposed a method for decellularizing porcine aortas by removing lipids from the aortas using liquefied dimethyl ether (DME) instead of the conventional sodium dodecyl sulfate (SDS). This is followed by DNA fragmentation with DNase. In the current work, the physical properties of porcine aortas decellularized using the DME method are evaluated by tensile strength tests. Conventional SDS decellularized aortas are typically swollen, rupture very easily, and have poor elasticity. By contrast, DME-treated samples are found to be less elastic. However, the maximum stress required for rupture is greater than that for the original aorta. These results indicate that decellularization with DME and DNase increases the maximum stress that can be withstood. Reduction of elasticity may derive from the appearance of temporary C=N bonds due to Schiff-base reactions that occur during the lipid removal process by liquefied DME, and methods to avoid this are desirable.