Integrated bioinformatics analysis for novel miRNAs markers and ceRNA network in diabetic retinopathy

Jingru Li; Chaozhong Li; Yulan Zhao; Xinyu Wu; Shuai Yu; Guihu Sun; Peng Ding; Si Lu; Lijiao Zhang; Ping Yang; Yunzhu Peng; Jingyun Fu; Luqiao Wang

doi:10.3389/fgene.2022.874885

Integrated bioinformatics analysis for novel miRNAs markers and ceRNA network in diabetic retinopathy

Front Genet. 2022 Sep 16:13:874885. doi: 10.3389/fgene.2022.874885. eCollection 2022.

Authors

Jingru Li¹, Chaozhong Li², Yulan Zhao³, Xinyu Wu¹, Shuai Yu¹, Guihu Sun¹, Peng Ding¹, Si Lu¹, Lijiao Zhang¹, Ping Yang¹, Yunzhu Peng¹, Jingyun Fu⁴, Luqiao Wang¹

Affiliations

¹ Department of Cardiology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
² Department of Emergency, The First Affiliated Hospital of Kunming Medical University, Kunming, China.
³ Department of Laboratory Animal Science, Kunming Medical University, Kunming, China.
⁴ Department of Endocrinology, The First Affiliated Hospital of Kunming Medical University, Kunming, China.

Abstract

In order to seek a more outstanding diagnosis and treatment of diabetic retinopathy (DR), we predicted the miRNA biomarkers of DR and explored the pathological mechanism of DR through bioinformatics analysis. Method: Based on public omics data and databases, we investigated ncRNA (non-coding RNA) functions based on the ceRNA hypothesis. Result: Among differentially expressed miRNAs (DE-miRNAs), hsa-miR-1179, -4797-3p and -665 may be diagnosis biomarkers of DR. Functional enrichment analysis revealed differentially expressed mRNAs (DE-mRNAs) enriched in mitochondrial transport, cellular respiration and energy derivation. 18 tissue/organ-specific expressed genes, 10 hub genes and gene cluster modules were identified. The ceRNA networks lncRNA FBXL19-AS1/miR-378f/MRPL39 and lncRNA UBL7-AS1/miR-378f/MRPL39 might be potential RNA regulatory pathways in DR. Conclusion: Differentially expressed hsa-miR-1179, -4797-3p and -665 can be used as powerful markers for DR diagnosis, and the ceRNA network: lncRNA FBXL19-AS1/UBL7-AS1-miR-378f-MRPL39 may represent an important regulatory role in DR progression.

Keywords: RNA regulatory pathways; bioinformatics analysis; biomarker; diabetic retinopathy; microarray.