Artificial chromosome technology and its potential application in plants

Manman Kan; Tengbo Huang; Panpan Zhao

doi:10.3389/fpls.2022.970943

Artificial chromosome technology and its potential application in plants

Front Plant Sci. 2022 Sep 15:13:970943. doi: 10.3389/fpls.2022.970943. eCollection 2022.

Authors

Manman Kan^{1

2}, Tengbo Huang¹, Panpan Zhao¹

Affiliations

¹ Guangdong Provincial Key Laboratory for Plant Epigenetics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong, China.
² Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, China.

Abstract

Plant genetic engineering and transgenic technology are powerful ways to study the function of genes and improve crop yield and quality in the past few years. However, only a few genes could be transformed by most available genetic engineering and transgenic technologies, so changes still need to be made to meet the demands for high throughput studies, such as investigating the whole genetic pathway of crop traits and avoiding undesirable genes simultaneously in the next generation. Plant artificial chromosome (PAC) technology provides a carrier which allows us to assemble multiple and specific genes to produce a variety of products by minichromosome. However, PAC technology also have limitations that may hinder its further development and application. In this review, we will introduce the current state of PACs technology from PACs formation, factors on PACs formation, problems and potential solutions of PACs and exogenous gene(s) integration.

Keywords: bottom-up; exogenous gene(s) integration; factors on PACs formation; plant artificial chromosome; problems and potential solutions of PACs; top-down.

Publication types

Review