Co-overexpression of AtSAT1 and EcPAPR improves seed nutritional value in maize

Xiaoli Xiang; Binhua Hu; Zhigang Pu; Lanying Wang; Thomas Leustek; Changsheng Li

doi:10.3389/fpls.2022.969763

Co-overexpression of AtSAT1 and EcPAPR improves seed nutritional value in maize

Front Plant Sci. 2022 Sep 15:13:969763. doi: 10.3389/fpls.2022.969763. eCollection 2022.

Authors

Xiaoli Xiang^{1

2}, Binhua Hu¹, Zhigang Pu¹, Lanying Wang¹, Thomas Leustek³, Changsheng Li²

Affiliations

¹ Institute of Biotechnology and Nuclear Technology, Sichuan Academy of Agricultural Sciences, Chengdu, China.
² The National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei, China.
³ Department of Plant Biology, Rutgers University, New Brunswick, NJ, United States.

Abstract

Maize seeds synthesize insufficient levels of the essential amino acid methionine (Met) to support animal and livestock growth. Serine acetyltransferase1 (SAT1) and 3'-phosphoadenosine-5'-phosphosulfate reductase (PAPR) are key control points for sulfur assimilation into Cys and Met biosynthesis. Two high-MET maize lines pRbcS:AtSAT1 and pRbcS:EcPAPR were obtained through metabolic engineering recently, and their total Met was increased by 1.4- and 1.57-fold, respectively, compared to the wild type. The highest Met maize line, pRbcS:AtSAT1-pRbcS:EcPAPR, was created by stacking the two transgenes, causing total Met to increase 2.24-fold. However, the pRbcS:AtSAT1-pRbcS:EcPAPR plants displayed progressively severe defects in plant growth, including early senescence, stunting, and dwarfing, indicating that excessive sulfur assimilation has an adverse effect on plant development. To explore the mechanism of correlation between Met biosynthesis in maize leaves and storage proteins in developing endosperm, the transcriptomes of the sixth leaf at stage V9 and 18 DAP endosperm of pRbcS:AtSAT1, pRbcS:AtSAT1-pRbcS:EcPAPR, and the null segregants were quantified and analyzed. In pRbcS:AtSAT1-pRbcS:EcPAPR, 3274 genes in leaves (1505 up- and 1769 downregulated) and 679 genes in the endosperm (327 up- and 352 downregulated) were differentially expressed. Gene ontology (GO) and KEGG (Kyoto encyclopedia of genes and genomes) analyses revealed that many genes were associated with Met homeostasis, including transcription factors and genes involved in cysteine and Met metabolism, glutathione metabolism, plant hormone signal transduction, and oxidation-reduction. The data from gene network analysis demonstrated that two genes, serine/threonine-protein kinase (CCR3) and heat shock 70 kDa protein (HSP), were localized in the core of the leaves and endosperm regulation networks, respectively. The results of this study provide insights into the diverse mechanisms that underlie the ideal establishment of enhanced Met levels in maize seeds.

Keywords: 3′-phosphoadenosine-5′-phosphosulfate reductase; methionine; protein–protein interaction; serine acetyltransferase1; transcriptome profiling analysis.