Biosynthesis of Lacto-N-fucopentaose I in Escherichia coli by metabolic pathway rational design

Miaomiao Hu; Mengli Li; Chenchen Li; Tao Zhang

doi:10.1016/j.carbpol.2022.120017

Biosynthesis of Lacto-N-fucopentaose I in Escherichia coli by metabolic pathway rational design

Carbohydr Polym. 2022 Dec 1:297:120017. doi: 10.1016/j.carbpol.2022.120017. Epub 2022 Aug 24.

Authors

Miaomiao Hu¹, Mengli Li¹, Chenchen Li¹, Tao Zhang²

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, Jiangsu 214122, China. Electronic address: zhangtao@jiangnan.edu.cn.

PMID: 36184139
DOI: 10.1016/j.carbpol.2022.120017

Abstract

Lacto-N-fucopentaose I (LNFP I), a member of the fucosylated human milk oligosaccharides (HMOs) family, has received widespread attention because of its importance in infant health. However, constructing a low-cost microbial cell factory for high-efficient production of complex fucosylated HMOs remains challenging. Herein, an efficient engineered strain for LNFP I biosynthesis was reported by metabolic pathway rational design in Escherichia coli BL21(DE3). The fed-batch cultivation of engineered strains produced 2.11 g/L LNFP I, and the conversion rate of lacto-N-tetraose (LNT) to LNFP I reached 32.55 %, the highest level reported so far. The LNFP I-produced platform established here is broadly suitable for complex and branched HMOs production.

Keywords: De novo pathway; Escherichia coli; Lacto-N-fucopentaose I; Metabolic engineering.

MeSH terms

Escherichia coli* / genetics
Escherichia coli* / metabolism
Humans
Metabolic Networks and Pathways
Milk, Human / metabolism
Oligosaccharides* / metabolism

Substances

Oligosaccharides
lacto-N-fucopentaose I