"Turn-off" photoelectrochemical aptasensor based on g-C3N4/WC/WO3 composites for tobramycin detection

Lu Qiao; Yuan Zhu; Tianjing Zeng; Yuanyuan Zhang; Mingjuan Zhang; Kexin Song; Nian Yin; Yani Tao; Yue Zhao; Yi Zhang; Chang Zhang

doi:10.1016/j.foodchem.2022.134287

"Turn-off" photoelectrochemical aptasensor based on g-C₃N₄/WC/WO₃ composites for tobramycin detection

Food Chem. 2023 Mar 1:403:134287. doi: 10.1016/j.foodchem.2022.134287. Epub 2022 Sep 17.

Authors

Lu Qiao¹, Yuan Zhu¹, Tianjing Zeng², Yuanyuan Zhang¹, Mingjuan Zhang¹, Kexin Song¹, Nian Yin¹, Yani Tao¹, Yue Zhao¹, Yi Zhang³, Chang Zhang⁴

Affiliations

¹ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, China.
² Hunan Ecological and Environmental Monitoring Center, Changsha 410001, China.
³ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, China. Electronic address: zyi@hnu.edu.cn.
⁴ College of Environmental Science and Engineering, Hunan University, Changsha 410082, China; Key Laboratory of Environmental Biology and Pollution Control, Hunan University, Ministry of Education, Changsha 410082, China. Electronic address: zhangchang@hnu.edu.cn.

PMID: 36183467
DOI: 10.1016/j.foodchem.2022.134287

Abstract

Tobramycin (TOB), as a widely used antibiotic, poses severe unpredictable risks to ecology and health. Here, a novel photoelectrochemical (PEC) aptasensor based on a "turn-off" PEC mode was constructed for TOB detection. The working electrode could be modified by g-C₃N₄/WC/WO₃ composites and TOB aptamer probes in turn. The TOB aptamer probes could anchor on the g-C₃N₄/WC/WO₃ through π-π stacking interaction to avoid interference from other modifications. When TOB was captured by the aptamer probes anchored on the modified fluorine-doped tin oxide (FTO) electrode, a decreased photocurrent was obtained owing to steric hindrance and hinder electron transfer. Under optimal conditions, 0.005-5 ng mL^-1 of TOB could be detected with a detection limit as low as 2 pg mL^-1. Meanwhile, actual samples were investigated as well. The proposed sensor shows high specificity, satisfactory detectability, great reproducibility, and may provide a new thought for detecting other pollutants.

Keywords: Aptamer probes; Photoelectrochemical biosensor; Tobramycin detection; g-C(3)N(4)/WC/WO(3) composites.

MeSH terms

Aptamers, Nucleotide*
Biosensing Techniques*
Electrochemical Techniques
Electrodes
Limit of Detection
Reproducibility of Results
Tobramycin

Substances

Aptamers, Nucleotide
Tobramycin