Generation of an S100B homozygous knockout pluripotent stem cell line (WAe009-A-94) by the CRISPR/Cas9 system

Pengxia Zhang; Hongyue Wang; Shengzhen Li; Tianwei Guo; Rui Zhang; Ping Zhou; Shaoli Ding

doi:10.1016/j.scr.2022.102924

Generation of an S100B homozygous knockout pluripotent stem cell line (WAe009-A-94) by the CRISPR/Cas9 system

Stem Cell Res. 2022 Oct:64:102924. doi: 10.1016/j.scr.2022.102924. Epub 2022 Sep 20.

Authors

Pengxia Zhang¹, Hongyue Wang², Shengzhen Li¹, Tianwei Guo³, Rui Zhang⁴, Ping Zhou⁵, Shaoli Ding⁶

Affiliations

¹ School of Stomatology, Lanzhou University, Lanzhou 730000, China.
² State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, Key Laboratory of Application of Pluripotent Stem Cells in Heart Regeneration, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100037, China.
³ Beijing Lab for Cardiovascular Precision Medicine, Anzhen Hospital, Capital Medical University, Beijing 100029, China.
⁴ School of Stomatology, Lanzhou University, Lanzhou 730000, China. Electronic address: zhangrui@lzu.edu.cn.
⁵ School of Stomatology, Lanzhou University, Lanzhou 730000, China. Electronic address: zhoup@lzu.edu.cn.
⁶ Department of Pain Treatment, Gansu Provincial Hospital, Lanzhou 730000, China. Electronic address: dingshl20@lzu.edu.cn.

PMID: 36182708
DOI: 10.1016/j.scr.2022.102924

Abstract

S100 calcium binding protein beta (S100B) is an S-100 low molecular weight binding protein that regulates intracellular processes. This protein is involved in myocardial contractility and calcium handling capacity. In this study, a human embryonic stem cell (hESC) line with homozygous S100B knockout (S100B-KO) was generated using the CRISPR/Cas9 editing system. This S100B-KO hESC line maintained normal cell morphology and karyotype, expressed pluripotency markers, and could differentiate into cells of all three germ layers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems* / genetics
Calcium / metabolism
Calcium-Binding Proteins
Homozygote
Human Embryonic Stem Cells* / metabolism
Humans
S100 Calcium Binding Protein beta Subunit / genetics
S100 Calcium Binding Protein beta Subunit / metabolism

Substances

Calcium
Calcium-Binding Proteins
S100B protein, human
S100 Calcium Binding Protein beta Subunit