To enable the utilization of seasonal biomasses in e.g., farm-scale biogas plants, the process should be flexible and ensure stable gas production. However, information about microbial community dynamics in long-term co-digestion with versatile co-feedstocks is lacking. This study investigated the effects of co-feedstock changes on the performance and evolution of microbial consortia during 428-day anaerobic digestion of cow slurry. Co-feedstocks consisted of hydrocarbon-, protein- and lipid-rich materials. A high throughput 16S ribosomal RNA gene sequencing was used to analyze the taxonomic profile of microbial communities. Due to the low loading rate, the changes were subtle in bacteria, but a shift on archaeal genera in response to different and changing feedstock compositions was observed. Despite drastic changes in co-feedstock composition, stable and flexible anaerobic digestion with relatively constant core microbiome can be achieved with cautious operation of the process.
Keywords: Anaerobic digester; Co-digestion; Methanogenesis; Microbiome; Substrates.
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