Analysis of the Human Pineal Proteome by Mass Spectrometry

Mariette Matondo; Guillaume Dumas; Erik Maronde

doi:10.1007/978-1-0716-2593-4_16

Analysis of the Human Pineal Proteome by Mass Spectrometry

Methods Mol Biol. 2022:2550:123-132. doi: 10.1007/978-1-0716-2593-4_16.

Authors

Mariette Matondo¹, Guillaume Dumas^{2

3}, Erik Maronde⁴

Affiliations

¹ Institut Pasteur, Université de Paris, CNRS USR2000, Proteomics Platform, Mass Spectrometry for Biology Unit, Paris, France.
² Institut Pasteur, UMR 3571 CNRS, University Paris Diderot, Paris France, Human Genetics and Cognitive Functions, Paris, France.
³ Computational Psychiatry, Department of Psychiatry and Addiction, University of Montreal, Montreal, Canada.
⁴ Institute for Anatomy II, Faculty of Medicine, Goethe University, Frankfurt, Germany. e.maronde@em.uni-frankfurt.de.

PMID: 36180685
DOI: 10.1007/978-1-0716-2593-4_16

Abstract

The human pineal gland regulates the day-night dynamics of multiple physiological processes, especially through the secretion of melatonin. Recently, using mass spectrometry-based proteomics and dedicated analysis tools, we have identified regulated proteins and signaling pathways that differ between day and night and/or between control and autistic pineal glands. This large-scale proteomic approach is the method of choice to study proteins in a biological system globally. This chapter proposes a protocol for large-scale analysis of the pineal gland proteome.

Keywords: Autism; Human; Mass spectrometry; Pineal gland; Protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Circadian Rhythm / physiology
Humans
Mass Spectrometry
Melatonin* / metabolism
Pineal Gland* / metabolism
Proteome / metabolism
Proteomics / methods

Substances

Proteome
Melatonin