Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland

Martin Fredensborg Rath; Morten Møller

doi:10.1007/978-1-0716-2593-4_10

Radiochemical In Situ Hybridization in Developmental Studies of the Pineal Gland

Methods Mol Biol. 2022:2550:75-84. doi: 10.1007/978-1-0716-2593-4_10.

Authors

Martin Fredensborg Rath¹, Morten Møller²

Affiliations

¹ Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark. mrath@sund.ku.dk.
² Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark.

PMID: 36180679
DOI: 10.1007/978-1-0716-2593-4_10

Abstract

Radiochemical in situ hybridization enables detection of gene expression in small areas of the brain, such as the developing pineal gland in rodents. The method combines determination of spatial and temporal gene expression profiles with semiquantitative analyses. We here describe the procedure of radiochemical in situ hybridization on the developing rat pineal gland ranging from preparation of fetal tissue for in situ hybridization to principles of quantification.

Keywords: Brain development; In situ hybridization; Pineal gland; Radioactive; Rodent.

MeSH terms

Animals
In Situ Hybridization
Pineal Gland* / chemistry
Pineal Gland* / metabolism
RNA, Messenger / metabolism
Rats

Substances

RNA, Messenger