EdU and Immunofluorescence Staining of Nothobranchius furzeri Organotypic Cultures

Cold Spring Harb Protoc. 2023 Mar 1;2023(3):107790-pdb.prot. doi: 10.1101/pdb.prot107790.

Abstract

Immunofluorescence is a widely used technique to visualize the localization of proteins of interest. Nucleoside analogs, such as 5-ethynyl-2'-deoxyuridine (EdU), are incorporated into newly synthesized DNA and enable permanent labeling of newly divided cells. Both these techniques can be applied to long-term organotypic culture of Nothobranchius furzeri in a fashion similar to that already described for tissue sections. We report here our optimized method for immunofluorescence and EdU staining of N. furzeri organotypic slices.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA*
  • Fluorescent Antibody Technique
  • Microphysiological Systems*
  • Staining and Labeling

Substances

  • DNA