Comparative study of five anti-lipopolysaccharide factor genes in Litopenaeus vannamei

Chenlin Yin; Xiaojing Shen; Yan Wang; Jingjie Hu; Zhenmin Bao; Mengqiang Wang

doi:10.1016/j.dci.2022.104557

Comparative study of five anti-lipopolysaccharide factor genes in Litopenaeus vannamei

Dev Comp Immunol. 2023 Feb:139:104557. doi: 10.1016/j.dci.2022.104557. Epub 2022 Sep 28.

Authors

Chenlin Yin¹, Xiaojing Shen¹, Yan Wang², Jingjie Hu³, Zhenmin Bao³, Mengqiang Wang⁴

Affiliations

¹ MOE Key Laboratory of Marine Genetics and Breeding, And Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institution, Qingdao 266003, Sanya, 572024, Ocean University of China, China.
² MOE Key Laboratory of Marine Genetics and Breeding, And Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institution, Qingdao 266003, Sanya, 572024, Ocean University of China, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572024, China.
³ MOE Key Laboratory of Marine Genetics and Breeding, And Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institution, Qingdao 266003, Sanya, 572024, Ocean University of China, China; Laboratory for Marine Fisheries Science and Food Production Processes, And Center for Marine Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572024, China.
⁴ MOE Key Laboratory of Marine Genetics and Breeding, And Key Laboratory of Tropical Aquatic Germplasm of Hainan Province of Sanya Oceanographic Institution, Qingdao 266003, Sanya, 572024, Ocean University of China, China; Laboratory for Marine Fisheries Science and Food Production Processes, And Center for Marine Molecular Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Hainan Yazhou Bay Seed Laboratory, Sanya, 572024, China. Electronic address: wangmengqiang@ouc.edu.cn.

PMID: 36179895
DOI: 10.1016/j.dci.2022.104557

Abstract

Anti-lipopolysaccharide factors (ALFs) are a family of common innate immune effectors in crustaceans, and they exhibit broad spectrum antimicrobial activity. In this study, we identified and characterized five novel ALF genes (designated as LvALF1-5) from the Pacific white shrimp (Litopenaeus vannamei) to investigate their potential immune functions. The amino acid sequence alignments showed that LvALFs contained two conserved cysteine residues, a hydrophobic N-terminal region, and the conserved signature sequence W(T/K)CPG(S)WT(A). They all shared high similarity with previously reported ALFs and were clearly novel members of the ALF family. The mRNA transcripts of LvALFs were most highly expressed in hemocytes and the hepatopancreas. After shrimp were stimulated with Vibrio parahaemolyticus or white spot syndrome virus, expression of the LvALFs was significantly induced in hemocytes and the hepatopancreas with various expression profiles. Recombinant proteins of LvALFs exhibited potent bacteriostatic activity in vitro. Together, these results suggest that LvALF1-5 participate in the immune response of Pacific white shrimp against invading pathogens.

Keywords: Anti-lipopolysaccharide factor; Innate immunity; Litopenaeus vannamei.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Lipopolysaccharides*

Substances

Lipopolysaccharides