Dialdehyde modification of laminarin for facile synthesis of ultrafine silver nanoparticles with excellent antibacterial and wound healing properties

Abstract

Laminarin is a promising marine biopolymer that is abundant, non-toxic, and biodegradable. However, laminarin has a weak reduction potential for metal ions, resulting in the synthesis of a lower content of large-sized silver nanoparticles (AgNPs). Here, we showed that after the introduction of aldehyde groups, the reduction potential of laminarin increased, decreasing the synthesis time and increasing the density of AgNPs. ¹H NMR and FT-IR confirmed the addition of aldehyde groups on laminarin. The dialdehyde-modified laminarin (DLAM) showed in situ, simple, and rapid synthesis of ultrasmall-sized spherical AgNPs (<10 nm), as revealed by TEM images. The aldehyde and carboxyl groups of DLAM act as synchronized reducing and anchoring agents. The conversion of Ag ions into AgNPs-DLAM was confirmed by UV-Vis spectrophotometer, FTIR, XRD, and XPS analysis. The AgNPs-DLAM showed significantly enhanced antibacterial activities than silver ions against Escherichia coli and Staphylococcus aureus via causing morphological changes and pore formations in bacterial cells. The AgNPs-DLAM also inhibited bacterial biofilm formation. In contrast, the AgNPs-DLAM showed negligible toxicity toward human keratinocytes. Furthermore, AgNPs-DLAM increased the migration of human keratinocytes, indicating efficient wound healing properties. Thus, signifying the importance of AgNPs-DLAM in clinical applications.

Keywords: Antimicrobial; Dialdehyde-modified laminarin; Silver nanoparticles; Ultrasmall-sized; Wound healing.