PROMIS: Co-fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions

Marcin Luzarowski; Ewelina M Sokolowska; Dennis Schlossarek; Aleksandra Skirycz

doi:10.1007/978-1-0716-2624-5_10

PROMIS: Co-fractionation Mass Spectrometry for Analysis of Protein-Metabolite Interactions

Methods Mol Biol. 2023:2554:141-153. doi: 10.1007/978-1-0716-2624-5_10.

Authors

Marcin Luzarowski^#¹, Ewelina M Sokolowska^#², Dennis Schlossarek^#², Aleksandra Skirycz³

Affiliations

¹ Zentrum für Molekulare Biologie der Universität Heidelberg, Potsdam, Germany.
² Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany.
³ Max Planck Institute of Molecular Plant Physiology, Potsdam, Germany. as4258@cornell.edu.

^# Contributed equally.

PMID: 36178625
DOI: 10.1007/978-1-0716-2624-5_10

Abstract

The roles of small molecules in every aspect of life have been gaining increased recognition. Many are known to exert their effect by binding proteins-but a comprehensive overview of protein-metabolite interactions (PMIs) is missing. Recently we devised a non-targeted method for detecting PMIs using size-exclusion chromatography followed by proteomic and metabolomic analysis: PROMIS. Under test this method was able to identify known PMIs such as enzyme-cofactor complexes as well as novel ones.

Keywords: Co-fractionation; Ligand; Mass spectrometry; Protein; Protein–metabolite interactions; Size exclusion chromatography.

Publication types

Review

MeSH terms

Chromatography, Gel
Mass Spectrometry / methods
Metabolomics
Proteins*
Proteomics* / methods

Substances

Proteins