Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

Yunfang Tian; Zhigang Chu; Linqing Wang; Huiyu Wang; Xiuyun Yuan; Si Wu; Yuzhen Yang

doi:10.2144/btn-2022-0025

Evaluation and validation of reference genes in Cymbidium faberi for real-time quantitative PCR

Biotechniques. 2022 Oct;73(4):171-181. doi: 10.2144/btn-2022-0025. Epub 2022 Sep 30.

Authors

Yunfang Tian^{1

2}, Zhigang Chu^{1

2}, Linqing Wang^{1

2}, Huiyu Wang^{1

2}, Xiuyun Yuan^{1

2}, Si Wu^{1

2}, Yuzhen Yang^{1

2}

Affiliations

¹ College of Life Sciences, Zhengzhou Normal University, Zhengzhou, 450044, People's Republic of China.
² Zhengzhou Key Laboratory of Ornamental and Medicinal Resources Plants, Zhengzhou, 450044, People's Republic of China.

PMID: 36178123
DOI: 10.2144/btn-2022-0025

Abstract

For all organs at all Cymbidium faberi stages, ACT, UBQ3 and GAPDH can be selected as reference genes. For organs of the vegetative stage, UBQ2 and UBQ3 can be chosen for analysis of normalized gene expression. For the bud stage, ACT and UBQ3 can be used for analysis of gene expression. For the full blossom stage, ACT, UBQ3 and UBQ2 can be introduced into relative gene expression analysis. For vegetative organs, UBQ2 and ACT can be used as reference genes. For reproductive organs, ACT, UBQ3 and UBQ2 can be used as a reference for data processing. CfAG1 gene expression is more consistent when UBQ3, GAPDH and ACT are used as reference genes.

Keywords: C. faberi; real-time quantitative PCR; reference genes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Flowers / genetics
Flowers / metabolism
Gene Expression Profiling
Genes, Plant*
Orchidaceae* / genetics
Orchidaceae* / metabolism
Real-Time Polymerase Chain Reaction
Reference Standards