Optical spectroscopy plays an important role in disease detection. Improving the sensitivity and specificity of spectral detection has great importance in the development of accurate diagnosis. The development of artificial intelligence technology provides a great opportunity to improve the detection accuracy through machine learning methods. In this Perspective, we focus on the combination of machine learning methods with the optical spectroscopy methods widely used for disease detection, including absorbance, fluorescence, scattering, FTIR, terahertz, etc. By comparing the spectral analysis with different machine learning methods, we illustrate that the support vector machine and convolutional neural network are most effective, which have potential to further improve the classification accuracy to distinguish disease subtypes if these machine learning methods are used. This Perspective broadens the scope of optical spectroscopy enhanced by machine learning and will be useful for the development of disease detection.