Protective effect of Limosilactobacillus fermentum HFY06 on dextran sulfate sodium-induced colitis in mice

Bihui Liu; Lei Yang; Ya Wu; Xin Zhao

doi:10.3389/fmicb.2022.935792

Protective effect of Limosilactobacillus fermentum HFY06 on dextran sulfate sodium-induced colitis in mice

Front Microbiol. 2022 Sep 12:13:935792. doi: 10.3389/fmicb.2022.935792. eCollection 2022.

Authors

Bihui Liu^{1

2

3

4}, Lei Yang⁵, Ya Wu^{1

2

3

4}, Xin Zhao^{1

2

3}

Affiliations

¹ Collaborative Innovation Center for Child Nutrition and Health Development, Chongqing University of Education, Chongqing, China.
² Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, China.
³ Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, China.
⁴ College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, China.
⁵ Department of Urology, First Affiliated Hospital of Chengdu Medical College, Chengdu, China.

Abstract

Ulcerative colitis is one of the main gastrointestinal diseases that threaten human health. This study investigated the effect of Limosilactobacillus fermentum HFY06 (LF-HFY06) on dextran sulfate sodium (DSS)-induced murine colitis. The protective effect of LF-HFY06 was evaluated by examining the length and histopathological sections of colon, related biochemical indicators, and genes related to inflammation. Direct and microscopic observations showed that LF-HFY06 increased the length of the colon and ameliorated the pathological damage induced by DSS. The biochemical indicators showed that LF-HFY06 enhanced the activities of antioxidant enzymes total superoxide dismutase (T-SOD) and catalase (CAT) in serum, while reducing the level of malondialdehyde (MDA). It was also observed that the serum inflammatory cytokines levels of tumor necrosis factor-α (TNF-α), interferon (IFN)-γ, interleukin (IL)-1β, IL-6, and IL-12 were decreased, and the anti-inflammatory cytokine IL-10 level was increased. The qPCR experiment revealed that LF-HFY06 downregulated the mRNA expression levels of nuclear factor-κB-p65 (Rela), Tnf, Il 1b, Il 6, and prostaglandin-endoperoxide synthase 2 (Ptgs2) in colon tissues, and upregulated the mRNA expression of NF-κB inhibitor-α (Nfkbia) and Il 10. These data indicated that LF-HFY06 inhibited inflammation through the NF-κB signaling pathway to prevent the occurrence and development of colitis. This research demonstrates that probiotics LF-HFY06 have the potential to prevent and treat colitis.

Keywords: Lactobacillus fermentum; colitis; dextran sulfate sodium (DSS); inflammation; probiotics.