Application of P/VB staining to identify antemortem injury in a decomposed cadaver

Forensic Sci Med Pathol. 2023 Sep;19(3):464-467. doi: 10.1007/s12024-022-00533-x. Epub 2022 Sep 28.

Abstract

It is challenging in forensic pathology to determine whether an injury is formed antemortem or postmortem in putrefied tissues. Hematoxylin-eosin (HE) staining fails to work in highly decomposed corpses. Ponceau/Victoria blue B (P/VB) staining is usually used to show collagen fibers and muscles. Here, we used P/VB to show antemortem laceration injury on the scalp in a severely decomposed cadaver 1.5 years after death. This method is a simple and alternative method for the diagnosis of antemortem injury.

Keywords: Antemortem injury; Forensic pathology; Ponceau/Victoria blue B staining; Putrefaction.

MeSH terms

  • Cadaver
  • Forensic Pathology
  • Humans
  • Lacerations*
  • Postmortem Changes*
  • Staining and Labeling