Change of mechanical characteristics in spider silk capture threads after contact with prey

Abstract

Most spiders rely on specialized capture threads to subdue prey. Cribellate spiders use capture threads, whose adhesion is based on thousands of nanofibers instead of specialized glue. The nanofibers adhere due to van der Waals and hygroscopic forces, but the adhesion is strengthened by an interaction with the cuticular hydrocarbons (CHCs) covering almost all insects. The interaction between CHCs and cribellate threads becomes visible through migration of the CHCs into the thread even far beyond the point of contact. In this study, we were able to show that the migrated CHCs not only influence adhesion but also change the mechanical characteristics of the thread. While adhesion, extensibility and total energy decreased in threads treated with CHCs from different insects, we observed an increasing force required to break threads. Such mechanical changes could be beneficial for the spider: Upon the first impact of the insect in the web, it is important to absorb all the energy without breaking. Afterwards, a reduction in extensibility could cause the insect to stay closer to the web and thus become additionally entangled in neighboring threads. An increased tensile force would additionally ensure that for insects already in the web, it is even harder to free themselves. Taken together, all these changes make it unlikely that cribellate spiders reuse their capture threads, if not reacting rapidly and removing the prey insect before the CHCs can spread across the thread. STATEMENT OF SIGNIFICANCE: Cribellate spiders use capture threads that, unlike other spiders, consist of nanofibers and do not rely glue. Instead, prey adheres mainly because their surface compounds, so-called cuticular hydrocarbons (CHCs), interact with the thread, this way generating strong adhesion forces. Previous studies on biomechanics and adhesion of cribellate threads only dealt with artificial surfaces, neglecting any interaction with surface compounds. This study examines the dramatical mechanical changes of a cribellate thread after interaction with prey CHCs, showing modifications of the thread's extensibility, tensile force and total energy. Our results highlight the importance of studying mechanical properties of silk not only in an artificial context, but also in real life.

Keywords: Adhesion; Biomechanics; Cuticular hydrocarbons; Extensibility; Insect-prey; Predator-prey interactions; Silk mechanics; Spiders; Tensile force.