Ti3C2@Pd nanocatalytic amplification-polypeptide SERS/RRS/Abs trimode biosensoring platformfor ultratrace trinitrotoluene

Hongyan Bai; Guiqing Wen; Aihui Liang; Zhiliang Jiang

doi:10.1016/j.bios.2022.114743

Ti₃C₂@Pd nanocatalytic amplification-polypeptide SERS/RRS/Abs trimode biosensoring platformfor ultratrace trinitrotoluene

Biosens Bioelectron. 2022 Dec 1:217:114743. doi: 10.1016/j.bios.2022.114743. Epub 2022 Sep 22.

Authors

Hongyan Bai¹, Guiqing Wen², Aihui Liang³, Zhiliang Jiang⁴

Affiliations

¹ State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, 541004, China; College of Pharmacy, Guilin Medical University, Guilin, 541199, China.
² State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, 541004, China. Electronic address: gqwen@mailbox.gxnu.edu.cn.
³ State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, 541004, China.
⁴ State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China; Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Guangxi Normal University), Ministry of Education, Guilin, 541004, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin, 541004, China. Electronic address: zljiang@mailbox.gxnu.edu.cn.

PMID: 36166889
DOI: 10.1016/j.bios.2022.114743

Abstract

A new MXene supported Pd nanoparticles (Ti₃C₂@Pd) nanosol with good stability and strong catalysis was prepared by the two-step procedure. Experiment was found that Ti₃C₂@Pd could strongly catalyze the reduction of HAuCl₄ by H₂O₂ to produce gold nanoparticles (AuNPs), with strong surface enhanced Raman scattering (SERS), resonance Rayleigh scattering (RRS) and surface plasmon resonance absorption (Abs). Coupled this new SERS/RRS/Abs trimode nanocatalytic indicator reaction with specific TNT polypeptide (PT_TNT), a facile and selective trimode polypeptide biosensoring platform was established for the detection of ultratrace TNT, with a linear range of 1.1-66, 1.1-66 and 4.4-66 pmol/L TNT, and detection limit (DL) of 0.69, 0.97 and 3.36 pmol/L by SERS, RRS and Abs assay respectively. It has been used to detect TNT in wastewater and soil samples, with recovery of 98.7-106% and RSD of 6.22-8.77%. In addition, this biosensoring platform can be also used to assay glyphosate and estradiol, respectively.

Keywords: 2; 4; 6-trinitrotoluene; Biosensing platform; Polypeptide; SERS/RRS/Abs trimode reaction; Ti(3)C(2)@Pd nanoenzyme.

MeSH terms

Biosensing Techniques*
Estradiol
Gold
Hydrogen Peroxide
Metal Nanoparticles*
Peptides
Soil
Spectrum Analysis, Raman / methods
Titanium
Trinitrotoluene*
Wastewater

Substances

Peptides
Soil
Waste Water
Trinitrotoluene
Estradiol
Gold
Hydrogen Peroxide
Titanium