Interspecific spread of dsRNA mycoviruses in entomogenous fungi Beauveria spp

Siyu Ning; Qin Kang; Hongyu Liu; Yang Lu; Li Sui; Wenjing Xu; Wangpeng Shi; Qiyun Li; Zhengkun Zhang

doi:10.1016/j.virusres.2022.198933

Interspecific spread of dsRNA mycoviruses in entomogenous fungi Beauveria spp

Virus Res. 2022 Dec:322:198933. doi: 10.1016/j.virusres.2022.198933. Epub 2022 Sep 20.

Authors

Siyu Ning¹, Qin Kang², Hongyu Liu³, Yang Lu³, Li Sui³, Wenjing Xu³, Wangpeng Shi⁴, Qiyun Li⁵, Zhengkun Zhang⁶

Affiliations

¹ Institute of Plant Protection, Jilin Academy of Agricultural Sciences; Jilin Key Laboratory of Agricultural Microbiology; Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Gongzhuling 136100, Jilin Province, China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
² Institute of Plant Protection, Jilin Academy of Agricultural Sciences; Jilin Key Laboratory of Agricultural Microbiology; Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Gongzhuling 136100, Jilin Province, China; State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
³ Institute of Plant Protection, Jilin Academy of Agricultural Sciences; Jilin Key Laboratory of Agricultural Microbiology; Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Gongzhuling 136100, Jilin Province, China.
⁴ Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China.
⁵ Institute of Plant Protection, Jilin Academy of Agricultural Sciences; Jilin Key Laboratory of Agricultural Microbiology; Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Gongzhuling 136100, Jilin Province, China; Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China. Electronic address: qyli1225@126.com.
⁶ Institute of Plant Protection, Jilin Academy of Agricultural Sciences; Jilin Key Laboratory of Agricultural Microbiology; Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Gongzhuling 136100, Jilin Province, China. Electronic address: zzk1980@cjaas.com.

PMID: 36165923
DOI: 10.1016/j.virusres.2022.198933

Abstract

Mycoviruses can spread interspecifically and intraspecifically in plant pathogenic fungi, as well as spreading intraspecifically in entomogenous fungi, especially Beauveria bassiana. However, whether mycoviruses are common in Beauveria spp. and can spread interspecifically between Beauveria species are unclear. Herein, four Beauveria species, but not B. bassiana, were randomly selected for double stranded RNA (dsRNA) detection. Furthermore, two previously reported dsRNA mycoviruses from B. bassiana, BbCV-2 and BbPmV-4, were used to study the interspecific transmission among B. bassiana, B. amorpha, and B. aranearum, using hyphal anastomosis and a novel insect coinfection transmission method. The results showed that dsRNA mycoviruses exist universally in Beauveria spp. and could spread interspecifically between different Beauveria species. The transmission efficiency from B. bassiana to the other two Beauveria species was significantly higher than that of the reverse transmission. Both viruses could stably and vertically spread in B. amorpha and B. aranearum, which affected their growth rate and colony morphology.

Keywords: Beauveria; Biological traits; Interspecific transmission; Mycoviruses.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Beauveria* / genetics
Fungal Viruses* / genetics
Insecta
Moths*
RNA, Double-Stranded / genetics

Substances

RNA, Double-Stranded