Bufogarlides A-C, three new bufadienolides with Δ14,15 double bond from the skins of Bufo bufo gargarizans

Lingjie Meng; Qinghong Kong; Jianxun Xie; Feng Pan; Keyu Lu; Sanhua Li; Yiling Li; Di Zhang; Yun Liu

doi:10.1080/14786419.2022.2127709

Bufogarlides A-C, three new bufadienolides with Δ^14,15 double bond from the skins of Bufo bufo gargarizans

Nat Prod Res. 2024 Jan-Feb;38(3):470-476. doi: 10.1080/14786419.2022.2127709. Epub 2022 Sep 27.

Authors

Lingjie Meng^{1

2}, Qinghong Kong^{1

2}, Jianxun Xie¹, Feng Pan^{1

2}, Keyu Lu^{1

2}, Sanhua Li^{1

2}, Yiling Li^{1

2}, Di Zhang^{1

2}, Yun Liu^{1

2

3}

Affiliations

¹ Guizhou Provincial College-based Key Lab for Tumor Prevention and Treatment with Distinctive Medicines, Zunyi Medical University, Zunyi, China.
² College of Basic Medicine, Zunyi Medical University, Zunyi, China.
³ School of Forensic Medicine, Zunyi Medical University, Zunyi, China.

PMID: 36165716
DOI: 10.1080/14786419.2022.2127709

Abstract

Three new bufadienolides with a Δ^14,15 double bond, named bufogarlides A-C (1-3), together with three known analogs (4-6), were isolated from the skins of Bufo bufo gargarizans. Their structures were identified by analyses of spectroscopic data (1 D and 2 D NMR, HR-ESIMS), and comparison with the literature data. All the isolates were evaluated for their cytotoxic activities against ovarian carcinoma cell lines A2780 and SKOV3. Among them, compound 5 showed the highest potential for the growth inhibition of cancerous cells A2780 and SKOV3 with the IC₅₀ values of 21.09 and 67.08 nM, respectively.

Keywords: Bufadienolides; Bufo bufo gargarizans; bufogarlides A-C; ovarian carcinoma cell lines.

MeSH terms

Animals
Bufanolides* / chemistry
Bufanolides* / pharmacology
Bufo bufo
Cell Line, Tumor
Female
Humans
Molecular Structure
Ovarian Neoplasms*

Substances

Bufanolides