Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage

Sivan David; Elena Levin; Elazar Fallik; Sharon Alkalai-Tuvia; Majid R Foolad; Amnon Lers

doi:10.3389/fpls.2022.991983

Physiological genetic variation in tomato fruit chilling tolerance during postharvest storage

Front Plant Sci. 2022 Sep 8:13:991983. doi: 10.3389/fpls.2022.991983. eCollection 2022.

Authors

Sivan David^{1

2}, Elena Levin¹, Elazar Fallik¹, Sharon Alkalai-Tuvia¹, Majid R Foolad³, Amnon Lers¹

Affiliations

¹ Department of Postharvest Science, Volcani Institute, Agricultural Research Organization, Rishon LeZion, Israel.
² Robert H. Smith Faculty of Agriculture Food and Environment, The Robert H. Smith Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel.
³ Department of Plant Science, The Pennsylvania State University, University Park, PA, United States.

Abstract

Storage at low temperatures is a common practice to prolong postharvest life of fruit and vegetables with a minimal negative impact on human/environmental health. Storage at low temperatures, however, can be restricted due to produce susceptibility to non-freezing chilling temperatures, when injuries such as physiological disorders and decays may result in unmarketable produce. We have investigated tomato fruit response to postharvest chilling stress in a recombinant inbred line (RIL) population developed from a cross between a chilling-sensitive cultivated tomato (Solanum lycopersicum L.) breeding line and a chilling-tolerant inbred accession of the tomato wild species S. pimpinellifolium L. Screening of the fruit of 148 RILs under cold storage (1.5°C) indicated presence of significant variations in chilling tolerance, manifested by varying degrees of fruit injury. Two extremely contrasting groups of RILs were identified, chilling-tolerant and chilling-sensitive RILs. The RILs in the two groups were further investigated under chilling stress conditions, and several physiological parameters, including weight loss, chlorophyll fluorescence parameters Fv/Fm, and Performance Index (PI), were determined to be efficient markers for identifying response to chilling stress in postharvest fruit. The Fv/Fm values reflected the physiological damages endured by the fruit after cold storage, and PI was a sensitive marker for early changes in photosystem II function. These two parameters were early indicators of chilling response before occurrence of visible chilling injuries. Antioxidant activities and ascorbic acid content were significantly higher in the chilling-tolerant than the chilling-sensitive lines. Further, the expression of C-repeat/DREB binding factors (CBFs) genes swiftly changed within 1-hr of fruit exposure to the chilling temperature, and the SlCBF1 transcript level was generally higher in the chilling-tolerant than chilling-sensitive lines after 2-hr exposure to the low temperature. This research demonstrates the presence of potential genetic variation in fruit chilling tolerance in the tomato RIL population. Further investigation of the RIL population is underway to better understand the genetic, physiological, and biochemical mechanisms involved in postharvest fruit chilling tolerance in tomato.

Keywords: Solanum lycopersicum; Solanum pimpinellifolium; antioxidant potential; chlorophyll fluorescence; cold storage; postharvest chilling tolerance.