Chemical inhibition of oxygen-sensing prolyl hydroxylases impairs angiogenic competence of human vascular endothelium through metabolic reprogramming

Ratnakar Tiwari; Prashant V Bommi; Peng Gao; Matthew J Schipma; Yalu Zhou; Susan E Quaggin; Navdeep S Chandel; Pinelopi P Kapitsinou

doi:10.1016/j.isci.2022.105086

Chemical inhibition of oxygen-sensing prolyl hydroxylases impairs angiogenic competence of human vascular endothelium through metabolic reprogramming

iScience. 2022 Sep 6;25(10):105086. doi: 10.1016/j.isci.2022.105086. eCollection 2022 Oct 21.

Authors

Ratnakar Tiwari^{1

2}, Prashant V Bommi^{1

2}, Peng Gao³, Matthew J Schipma⁴, Yalu Zhou^{1

2}, Susan E Quaggin^{1

2}, Navdeep S Chandel³, Pinelopi P Kapitsinou^{1

2

3}

Affiliations

¹ Feinberg Cardiovascular Research Institute, Northwestern University Feinberg School of Medicine, 303 East Superior Street, SQBRC 8-408, Chicago, 60611 IL, USA.
² Division of Nephrology & Hypertension, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
³ Department of Medicine and Robert H. Lurie Cancer Center, Northwestern University Feinberg School of Medicine, Chicago, IL, USA.
⁴ Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.

Abstract

Endothelial cell (EC) metabolism has emerged as a driver of angiogenesis. While hypoxia inactivates the oxygen sensors prolyl-4 hydroxylase domain-containing proteins 1-3 (PHD1-3) and stimulates angiogenesis, the effects of PHDs on EC functions remain poorly defined. Here, we investigated the impact of chemical PHD inhibition by dimethyloxalylglycine (DMOG) on angiogenic competence and metabolism of human vascular ECs. DMOG reduced EC proliferation, migration, and tube formation capacities, responses that were associated with an unfavorable metabolic reprogramming. While glycolytic genes were induced, multiple genes encoding sub-units of mitochondrial complex I were suppressed with concurrent decline in nicotinamide adenine dinucleotide (NAD⁺) levels. Importantly, the DMOG-induced defects in EC migration could be partially rescued by augmenting NAD⁺ levels through nicotinamide riboside or citrate supplementation. In summary, by integrating functional assays, transcriptomics, and metabolomics, we provide insights into the effects of PHD inhibition on angiogenic competence and metabolism of human vascular ECs.

Keywords: Biological sciences; Metabolomics; Transcriptomics.

Abstract

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