Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis

Methods Mol Biol. 2023:2570:271-280. doi: 10.1007/978-1-0716-2695-5_20.

Abstract

Electrochemical aptamer-based (E-AB) sensors using conformational change-induced electron transfer kinetics are sensitive, reagent-less, and cost-effective tools for molecular sensing. Current advances in this technology can allow continuous drug pharmacokinetic monitoring in living animals (Dauphin-Ducharme et al., ACS Sens 4(10):2832-2837, 2019; Idili et al., Chem Sci 10(35):8164-8170, 2019), as well as automated analysis of hormone pulsatility (Liang et al., Nat Commun 10(1):852, 2019). In this chapter, we provide the methodology for an automated E-AB conformational change-based robotic sensing platform. By using an open-source programmable robotic system, this method can be adapted to a wide range of experimental scenarios.

Keywords: Aptamer; Aptasensor; Automated analysis; Biosensor; Electrochemical aptamer-based; Liquid-handling robot; Luteinizing hormone; Opentron; RAPTER; Squarewave voltammetry.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aptamers, Nucleotide* / chemistry
  • Biosensing Techniques* / methods
  • Electrochemical Techniques / methods
  • Electrodes
  • Gold / chemistry
  • Hormones
  • Robotic Surgical Procedures*

Substances

  • Aptamers, Nucleotide
  • Hormones
  • Gold