Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT

Sebastian Strauss; Ralf Jungmann

doi:10.1007/978-1-0716-2695-5_13

Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT

Methods Mol Biol. 2023:2570:177-185. doi: 10.1007/978-1-0716-2695-5_13.

Authors

Sebastian Strauss^{1

2}, Ralf Jungmann^{3

4}

Affiliations

¹ Max Planck Institute of Biochemistry, Martinsried, Germany.
² Faculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany.
³ Max Planck Institute of Biochemistry, Martinsried, Germany. jungmann@biochem.mpg.de.
⁴ Faculty of Physics and Center for Nanoscience, Ludwig Maximilian University, Munich, Germany. jungmann@biochem.mpg.de.

PMID: 36156782
DOI: 10.1007/978-1-0716-2695-5_13

Abstract

Super-resolution microscopy methods enable the visualization of biological processes on the level of a few nanometers. However, the application of these techniques in biological systems is limited by the availability of small affinity reagents. Slow off-rate-modified aptamers as nucleic acid analogues to antibodies have been successfully applied to improve the resolution and quantification of DNA-PAINT. In this chapter, we describe a protocol for using SOMAmers as labeling reagents for super-resolution microscopy.

Keywords: Aptamer; DNA-PAINT; Fluorescence microscopy; Labeling probes; SOMAmer; Super-resolution.

MeSH terms

DNA
Microscopy, Fluorescence / methods
Nucleic Acids*
Oligonucleotides*

Substances

Nucleic Acids
Oligonucleotides
DNA