Automated ssDNA SELEX Using Histidine-Tagged Target Proteins

Stefan Breuers; Günter Mayer

doi:10.1007/978-1-0716-2695-5_1

Automated ssDNA SELEX Using Histidine-Tagged Target Proteins

Methods Mol Biol. 2023:2570:3-11. doi: 10.1007/978-1-0716-2695-5_1.

Authors

Stefan Breuers^{1

2}, Günter Mayer^{3

4}

Affiliations

¹ Life and Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany.
² Center of Aptamer Research and Development, University of Bonn, Bonn, Germany.
³ Life and Medical Sciences Institute (LIMES), University of Bonn, Bonn, Germany. gmayer@uni-bonn.de.
⁴ Center of Aptamer Research and Development, University of Bonn, Bonn, Germany. gmayer@uni-bonn.de.

PMID: 36156770
DOI: 10.1007/978-1-0716-2695-5_1

Abstract

The systematic evolution of ligands by exponential enrichment (SELEX) enables the identification of ssDNA or RNA sequences binding to different target molecules, highly specific and with high affinity. In this chapter, we describe a selection strategy with ssDNA for a histidine-tagged protein that could be either performed hands-on manually or fully automated by an appropriate robotic selection platform.

Keywords: Aptamer; Automated in vitro selection; Gel electrophoresis; Protein immobilization; SELEX; ssDNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Nucleotide* / chemistry
DNA, Single-Stranded / genetics
Histidine / genetics
Ligands
Proteins / genetics
SELEX Aptamer Technique*

Substances

Aptamers, Nucleotide
DNA, Single-Stranded
Ligands
Proteins
Histidine