Hyaluronidase (HAase) can depolymerize mucopolysaccharide hyaluronic acid (HA) to increase the efficacy of drug diffusion in the case of pathogenic bacteria. Due to its widespread medical applications, HAase originating from microorganisms has attracted significant attention. In this study, the HAase-producing bacterium Bacillus sp. CQMU-D was isolated from soil and identified based on its morphology and 16S rRNA gene sequencing. Enzyme activity was detected by measuring the content of reducing sugar in HA degradation products with 3,5-dinitrosalicylic acid (DNSA) or p-dimethylaminobenzaldehyde (DMAB) as the detection reagent. The results revealed that HAase reached maximum activity after 48 h of cultivation. Gene function annotation after full-length sequencing showed increased transport and metabolic activities associated with HAase. Additionally, the HAase gene of Bacillus sp. CQMU-D was different from the existing microbial HAase, and its protein was predicted to be a stable secretory protein with a conserved GAG_Lyase domain. These results characterized a new HAase-producing Bacillus from the soil via enzyme activity and bioinformatic analysis, expanding the knowledge on Bacillus HAase for potential industrial applications.
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