Antiviral activity of canine interferon lambda 3 expressed using a recombinant adenovirus against canine coronavirus, canine parvovirus, and canine distemper virus

Vet Res Commun. 2022 Dec;46(4):1363-1368. doi: 10.1007/s11259-022-10000-1. Epub 2022 Sep 26.

Abstract

Canine coronavirus (CCoV), canine parvovirus (CPV), and canine distemper virus (CDV) are highly contagious canine pathogens; dogs with these diseases are difficult to treat. In a previous study, we developed a recombinant adenovirus expressing canine interferon lambda 3 (Ad-caIFNλ3) in canine epithelial cells. In this study, we aimed to investigate the antiviral activity of Ad-caIFNλ3 against CCoV, CPV, and CDV in two canine cell lines, A72 and MDCK. Ad-caIFNλ3 transduction suppressed replication of these viruses without cytotoxicity. Our results suggest that Ad-caIFNλ3 may be a therapeutic candidate for canine viral diseases.

Keywords: Canine coronavirus (CCoV); Canine distemper virus (CDV); Canine interferon lambda 3; Canine parvovirus (CPV); In vitro; Recombinant adenovirus; Therapeutics.

MeSH terms

  • Adenoviridae
  • Adenoviridae Infections* / veterinary
  • Animals
  • Antibodies, Viral
  • Antiviral Agents
  • Coronavirus, Canine* / genetics
  • Distemper Virus, Canine* / genetics
  • Distemper*
  • Dog Diseases*
  • Dogs
  • Parvoviridae Infections* / veterinary
  • Parvovirus, Canine* / genetics

Substances

  • interferon-lambda, human
  • Antiviral Agents
  • Antibodies, Viral