Complex acoustic systems typically present three-dimensional distributions of noise sources. Conventional acoustic imaging methods with planar microphone arrays are unsuitable for three-dimensional acoustic imaging, given the computational demands and the incapability to explicitly account for the presence of multiple sources. This paper proposes the use of global optimization methods to solve these shortcomings. An experiment with three incoherent speakers proved that this method can accurately determine the three-dimensional location and the respective sound level of each individual source. In addition, super-resolution is achieved beyond half the Rayleigh resolution limit.